Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars

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Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars. / Smolikova, Galina; Shiroglazova, Olga; Vinogradova, Galina; Leppyanen, Irina; Dinastiya, Ekaterina; Yakovleva, Olga; Dolgikh, Elena; Titova, Galina; Frolov, Andrej; Medvedev, Sergei.

In: Functional Plant Biology, Vol. 47, No. 5, 2020, p. 409-424.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{24b2752cbf884c65b0f3460660864dc6,

title = "Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars",

abstract = "Developing seeds of some higher plants are photosynthetically active and contain chlorophylls (Chl), which are typically destroyed at the late stages of seed maturation. However, in some crop plant cultivars, degradation of embryonic Chl remains incomplete, and mature seeds preserve green colour, as it is known for green-seeded cultivars of pea (Pisum sativum L.). The residual Chl compromise seed quality and represent a severe challenge for farmers. Hence, comprehensive understanding of the molecular mechanisms, underlying incomplete Chl degradation is required for maintaining sustainable agriculture. Therefore, here we address dynamics of plastid conversion and photochemical activity alterations, accompanying degradation of Chl in embryos of yellow- and green-seeded cultivars Frisson and Rondo respectively. The yellow-seeded cultivar demonstrated higher rate of Chl degradation at later maturation stage, accompanied with termination of photochemical activity, seed dehydration and conversion of green plastids into amyloplasts. In agreement with this, expression of genes encoding enzymes of Chl degradation was lower in the green seeded cultivar, with the major differences in the levels of Chl b reductase (NYC1) and pheophytinase (PPH) transcripts. Thus, the difference between yellow and green seeds can be attributed to incomplete Chl degradation in the latter at the end of maturation period.",

keywords = "chlorophylls, chloroplasts, photosynthesis, seed development, embryogenesis, Pisum spp., chlorophylls, chloroplasts, embryogenesis, Pisum spp., photosynthesis, seed development, Pisum spp, IDENTIFICATION, REGULATOR, METABOLISM, BIOCHEMISTRY, GENE-EXPRESSION, STAY-GREEN, ARABIDOPSIS, COTYLEDON GENE, GRADIENTS, PHOTOSYNTHESIS",

author = "Galina Smolikova and Olga Shiroglazova and Galina Vinogradova and Irina Leppyanen and Ekaterina Dinastiya and Olga Yakovleva and Elena Dolgikh and Galina Titova and Andrej Frolov and Sergei Medvedev",

note = "Funding Information: The part of the study dealing with the light and transmission electron microscopy and Chl fluorescence analysis was supported by the Russian Science Foundation (50% of the study; Grant 16-16-00026 to S.M.). Experiments with qRT–PCR analysis and critical analyses of data, including manuscript preparation, were funded by Russian Science Foundation (50% of study; Grant 17-16-01042 to A.F.). Thanks to the Research Resource Centre for Molecular and Cell Technologies of Saint Petersburg State University and the Core Facility Centre {\textquoteleft}Cell and Molecular Technologies in Plant Science{\textquoteright} at the Komarov Botanical Institute of Russian Academy of Sciences (Saint Petersburg, Russia) for technical support.",

year = "2020",

doi = "10.1071/FP19270",

language = "English",

volume = "47",

pages = "409--424",

journal = "Functional Plant Biology",

issn = "1445-4408",

publisher = "CSIRO",

number = "5",

}

RIS

TY - JOUR

T1 - Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars

AU - Smolikova, Galina

AU - Shiroglazova, Olga

AU - Vinogradova, Galina

AU - Leppyanen, Irina

AU - Dinastiya, Ekaterina

AU - Yakovleva, Olga

AU - Dolgikh, Elena

AU - Titova, Galina

AU - Frolov, Andrej

AU - Medvedev, Sergei

N1 - Funding Information: The part of the study dealing with the light and transmission electron microscopy and Chl fluorescence analysis was supported by the Russian Science Foundation (50% of the study; Grant 16-16-00026 to S.M.). Experiments with qRT–PCR analysis and critical analyses of data, including manuscript preparation, were funded by Russian Science Foundation (50% of study; Grant 17-16-01042 to A.F.). Thanks to the Research Resource Centre for Molecular and Cell Technologies of Saint Petersburg State University and the Core Facility Centre ‘Cell and Molecular Technologies in Plant Science’ at the Komarov Botanical Institute of Russian Academy of Sciences (Saint Petersburg, Russia) for technical support.

PY - 2020

Y1 - 2020

N2 - Developing seeds of some higher plants are photosynthetically active and contain chlorophylls (Chl), which are typically destroyed at the late stages of seed maturation. However, in some crop plant cultivars, degradation of embryonic Chl remains incomplete, and mature seeds preserve green colour, as it is known for green-seeded cultivars of pea (Pisum sativum L.). The residual Chl compromise seed quality and represent a severe challenge for farmers. Hence, comprehensive understanding of the molecular mechanisms, underlying incomplete Chl degradation is required for maintaining sustainable agriculture. Therefore, here we address dynamics of plastid conversion and photochemical activity alterations, accompanying degradation of Chl in embryos of yellow- and green-seeded cultivars Frisson and Rondo respectively. The yellow-seeded cultivar demonstrated higher rate of Chl degradation at later maturation stage, accompanied with termination of photochemical activity, seed dehydration and conversion of green plastids into amyloplasts. In agreement with this, expression of genes encoding enzymes of Chl degradation was lower in the green seeded cultivar, with the major differences in the levels of Chl b reductase (NYC1) and pheophytinase (PPH) transcripts. Thus, the difference between yellow and green seeds can be attributed to incomplete Chl degradation in the latter at the end of maturation period.

AB - Developing seeds of some higher plants are photosynthetically active and contain chlorophylls (Chl), which are typically destroyed at the late stages of seed maturation. However, in some crop plant cultivars, degradation of embryonic Chl remains incomplete, and mature seeds preserve green colour, as it is known for green-seeded cultivars of pea (Pisum sativum L.). The residual Chl compromise seed quality and represent a severe challenge for farmers. Hence, comprehensive understanding of the molecular mechanisms, underlying incomplete Chl degradation is required for maintaining sustainable agriculture. Therefore, here we address dynamics of plastid conversion and photochemical activity alterations, accompanying degradation of Chl in embryos of yellow- and green-seeded cultivars Frisson and Rondo respectively. The yellow-seeded cultivar demonstrated higher rate of Chl degradation at later maturation stage, accompanied with termination of photochemical activity, seed dehydration and conversion of green plastids into amyloplasts. In agreement with this, expression of genes encoding enzymes of Chl degradation was lower in the green seeded cultivar, with the major differences in the levels of Chl b reductase (NYC1) and pheophytinase (PPH) transcripts. Thus, the difference between yellow and green seeds can be attributed to incomplete Chl degradation in the latter at the end of maturation period.

KW - chlorophylls

KW - chloroplasts

KW - photosynthesis

KW - seed development

KW - embryogenesis

KW - Pisum spp.

KW - chlorophylls

KW - chloroplasts

KW - embryogenesis

KW - Pisum spp.

KW - photosynthesis

KW - seed development

KW - Pisum spp

KW - IDENTIFICATION

KW - REGULATOR

KW - METABOLISM

KW - BIOCHEMISTRY

KW - GENE-EXPRESSION

KW - STAY-GREEN

KW - ARABIDOPSIS

KW - COTYLEDON GENE

KW - GRADIENTS

KW - PHOTOSYNTHESIS

UR - http://www.scopus.com/inward/record.url?scp=85082462868&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/d25ad360-deae-3027-88ab-ec4600c5b8f2/

U2 - 10.1071/FP19270

DO - 10.1071/FP19270

M3 - Article

VL - 47

SP - 409

EP - 424

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 5

ER -

ID: 52490898