Research output: Contribution to journal › Article › peer-review
Comparative Analysis of HUVEC and EA.hy926 Functional Characteristics under the Influence of Anti-Endoglin Antibodies. / Stolbovaya, A. Y.; Smirnov, I. V.; Pinevich, A. A.; Vartanyan, N. L.; Krutetskaya, I. Y.; Terekhina, L. A.; Markova, K. L.; Malashicheva, A. B.; Samoilovich, M. P.
In: Cell and Tissue Biology, Vol. 15, No. 6, 01.11.2021, p. 503-517.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Comparative Analysis of HUVEC and EA.hy926 Functional Characteristics under the Influence of Anti-Endoglin Antibodies
AU - Stolbovaya, A. Y.
AU - Smirnov, I. V.
AU - Pinevich, A. A.
AU - Vartanyan, N. L.
AU - Krutetskaya, I. Y.
AU - Terekhina, L. A.
AU - Markova, K. L.
AU - Malashicheva, A. B.
AU - Samoilovich, M. P.
N1 - Publisher Copyright: © 2021, Pleiades Publishing, Ltd.
PY - 2021/11/1
Y1 - 2021/11/1
N2 - Abstract: Endoglin, a co-receptor of TGF-β family growth factors, is an endothelial cells marker. In our previous work we have demonstrated that monoclonal antibodies (MABs) against endoglin can change the functional properties of endothelial cells EA.hy926. The aim of the present work was to study the effects of these antibodies on HUVEC endothelial cells, as well as to correlate the data obtained for HUVEC and EA.hy926 cells. Comparison of in vitro models based on the permanent EA.hy926 endothelial cell line and primary HUVECs revealed, along with common morpho-functional properties, a number of dissimilarities in the le-vels of adhesion molecules and the expression of endothelial differentiation genes. Anti-endoglin MABs 2C8 and 4E4 inhibited HUVEC cells migration, reduced their adhesion to solid substrate, influenced the arrangement of actin microfilaments, and impeded the formation of caspillary-like structures. These effects were revealed either in presence of TGF-β1 in culture medium or under hypoxia. Supplementation of the growth medium with MABs 2C8 promoted endoglin shedding from HUVEC membrane both in hypoxia and normoxia. Several impacts of anti-endoglin MABs on HUVEC cultures were similar to those detected on EA.hy926 cells. However the effect of MABs 2C8 on endoglin shedding by HUVEC and EA.hy926 cells was opposite.
AB - Abstract: Endoglin, a co-receptor of TGF-β family growth factors, is an endothelial cells marker. In our previous work we have demonstrated that monoclonal antibodies (MABs) against endoglin can change the functional properties of endothelial cells EA.hy926. The aim of the present work was to study the effects of these antibodies on HUVEC endothelial cells, as well as to correlate the data obtained for HUVEC and EA.hy926 cells. Comparison of in vitro models based on the permanent EA.hy926 endothelial cell line and primary HUVECs revealed, along with common morpho-functional properties, a number of dissimilarities in the le-vels of adhesion molecules and the expression of endothelial differentiation genes. Anti-endoglin MABs 2C8 and 4E4 inhibited HUVEC cells migration, reduced their adhesion to solid substrate, influenced the arrangement of actin microfilaments, and impeded the formation of caspillary-like structures. These effects were revealed either in presence of TGF-β1 in culture medium or under hypoxia. Supplementation of the growth medium with MABs 2C8 promoted endoglin shedding from HUVEC membrane both in hypoxia and normoxia. Several impacts of anti-endoglin MABs on HUVEC cultures were similar to those detected on EA.hy926 cells. However the effect of MABs 2C8 on endoglin shedding by HUVEC and EA.hy926 cells was opposite.
KW - EA.hy926
KW - endoglin
KW - endothelial cells
KW - HUVEC
KW - monoclonal antibodies
UR - http://www.scopus.com/inward/record.url?scp=85120949409&partnerID=8YFLogxK
U2 - 10.1134/s1990519x21060109
DO - 10.1134/s1990519x21060109
M3 - Article
AN - SCOPUS:85120949409
VL - 15
SP - 503
EP - 517
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 6
ER -
ID: 89780062