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Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway. / Markov, Alexander G.; Falchuk, Evgeny L.; Kruglova, Natalia M.; Radloff, Judith; Amasheh, Salah.

In: Acta Physiologica, Vol. 216, No. 1, 2016, p. 112-119.

Research output: Contribution to journal › Article

Harvard

Markov, AG, Falchuk, EL, Kruglova, NM, Radloff, J & Amasheh, S 2016, 'Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway', Acta Physiologica, vol. 216, no. 1, pp. 112-119. https://doi.org/10.1111/apha.12559

APA

Markov, A. G., Falchuk, E. L., Kruglova, N. M., Radloff, J., & Amasheh, S. (2016). Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway. Acta Physiologica, 216(1), 112-119. https://doi.org/10.1111/apha.12559

Vancouver

Markov AG, Falchuk EL, Kruglova NM, Radloff J, Amasheh S. Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway. Acta Physiologica. 2016;216(1):112-119. https://doi.org/10.1111/apha.12559

Author

Markov, Alexander G. ; Falchuk, Evgeny L. ; Kruglova, Natalia M. ; Radloff, Judith ; Amasheh, Salah. / Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway. In: Acta Physiologica. 2016 ; Vol. 216, No. 1. pp. 112-119.

BibTeX

@article{95e39ce1083b45a296cb025f7f4461d0,
title = "Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway",
abstract = "Abstract AIM: Members of the tight junction protein family of claudins have been demonstrated to specifically determine paracellular permeability of the intestinal epithelium. In small intestinal mucosa, which is generally considered to be a leaky epithelium, Peyer's patches are a primary part of the immune system. Aim of the study was to analyze the tight junctional barrier of follicle-associated epithelium covering Peyer's patches lymphoid follicles. METHODS: Employing small intestinal tissue specimens of male Wistar rats, electrophysiological analyses including the Ussing chamber technique, marker flux measurements and one-path impedance spectroscopy were performed. Morphometry of HE stained tissue sections was taken into account. Claudin expression and localization was analyzed by immunoblotting and confocal laser scanning immunofluorescence microscopy. RESULTS: Almost two-fold higher parameters of epithelial and transepithelial tissue resistance, and a markedly lower permeability for the paracellular per",
keywords = "impedance spectroscopy, tissue barrier, tight junctions, villous epithelium",
author = "Markov, {Alexander G.} and Falchuk, {Evgeny L.} and Kruglova, {Natalia M.} and Judith Radloff and Salah Amasheh",
year = "2016",
doi = "10.1111/apha.12559",
language = "English",
volume = "216",
pages = "112--119",
journal = "Skandinavisches Archiv Für Physiologie",
issn = "0370-839X",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Claudin expression in follicle-associated epithelium of rat Peyer's patches defines a major restriction of the paracellular pathway

AU - Markov, Alexander G.

AU - Falchuk, Evgeny L.

AU - Kruglova, Natalia M.

AU - Radloff, Judith

AU - Amasheh, Salah

PY - 2016

Y1 - 2016

N2 - Abstract AIM: Members of the tight junction protein family of claudins have been demonstrated to specifically determine paracellular permeability of the intestinal epithelium. In small intestinal mucosa, which is generally considered to be a leaky epithelium, Peyer's patches are a primary part of the immune system. Aim of the study was to analyze the tight junctional barrier of follicle-associated epithelium covering Peyer's patches lymphoid follicles. METHODS: Employing small intestinal tissue specimens of male Wistar rats, electrophysiological analyses including the Ussing chamber technique, marker flux measurements and one-path impedance spectroscopy were performed. Morphometry of HE stained tissue sections was taken into account. Claudin expression and localization was analyzed by immunoblotting and confocal laser scanning immunofluorescence microscopy. RESULTS: Almost two-fold higher parameters of epithelial and transepithelial tissue resistance, and a markedly lower permeability for the paracellular per

AB - Abstract AIM: Members of the tight junction protein family of claudins have been demonstrated to specifically determine paracellular permeability of the intestinal epithelium. In small intestinal mucosa, which is generally considered to be a leaky epithelium, Peyer's patches are a primary part of the immune system. Aim of the study was to analyze the tight junctional barrier of follicle-associated epithelium covering Peyer's patches lymphoid follicles. METHODS: Employing small intestinal tissue specimens of male Wistar rats, electrophysiological analyses including the Ussing chamber technique, marker flux measurements and one-path impedance spectroscopy were performed. Morphometry of HE stained tissue sections was taken into account. Claudin expression and localization was analyzed by immunoblotting and confocal laser scanning immunofluorescence microscopy. RESULTS: Almost two-fold higher parameters of epithelial and transepithelial tissue resistance, and a markedly lower permeability for the paracellular per

KW - impedance spectroscopy

KW - tissue barrier

KW - tight junctions

KW - villous epithelium

U2 - 10.1111/apha.12559

DO - 10.1111/apha.12559

M3 - Article

VL - 216

SP - 112

EP - 119

JO - Skandinavisches Archiv Für Physiologie

JF - Skandinavisches Archiv Für Physiologie

SN - 0370-839X

IS - 1

ER -

ID: 7546377