TY - JOUR

T1 - Classification and identification tasks in microbiology: mass spectrometric methods coming to the aid

AU - Velichko, N. V.

AU - Pinevich, A. V.

N1 - Velichko, N.V. & Pinevich, A.V. Microbiology (2019) 88: 534. https://doi.org/10.1134/S0026261719050151

PY - 2019

Y1 - 2019

N2 - Mass spectrometry (MS) methods furnish the clue to many microbiological applications including advanced studies on the diversity and classification of prokaryotes. Mass spectral data contribute to the polyphasic taxonomy which considers genotypic characters together with structure-functional and ecological traits. Additionally, these methods contribute to reliable and rapid identification of microorganisms bypassing conventional manipulations which are materials and time consuming. MS based analyses of biomarkers can be performed at the level of whole cells, cell homogenates, subcellular fractions, and individual molecules. For this purpose, various MS methods can be employed, such as MALDI-TOF, ESI, SELDI, and BAMS. Of these, MALDI-TOF MS is the especially easy-to-use and rapid method with many analytical applications, primarily in proteomics which aims at comprehensive description of protein inventory in prokaryotes. An alternative for detection and comparison of biomarkers via MS is amplification and alignment of marker gene sequences. Two molecular approaches supplement each other under support of database resources. Microbiologists readily assimilate MS methods propelled by high performance analyzers and sensitive detectors. The review focuses at progressing application of MS methods in microbiology, with an emphasis on identification and comparative study of bacteria.

AB - Mass spectrometry (MS) methods furnish the clue to many microbiological applications including advanced studies on the diversity and classification of prokaryotes. Mass spectral data contribute to the polyphasic taxonomy which considers genotypic characters together with structure-functional and ecological traits. Additionally, these methods contribute to reliable and rapid identification of microorganisms bypassing conventional manipulations which are materials and time consuming. MS based analyses of biomarkers can be performed at the level of whole cells, cell homogenates, subcellular fractions, and individual molecules. For this purpose, various MS methods can be employed, such as MALDI-TOF, ESI, SELDI, and BAMS. Of these, MALDI-TOF MS is the especially easy-to-use and rapid method with many analytical applications, primarily in proteomics which aims at comprehensive description of protein inventory in prokaryotes. An alternative for detection and comparison of biomarkers via MS is amplification and alignment of marker gene sequences. Two molecular approaches supplement each other under support of database resources. Microbiologists readily assimilate MS methods propelled by high performance analyzers and sensitive detectors. The review focuses at progressing application of MS methods in microbiology, with an emphasis on identification and comparative study of bacteria.

KW - BAMS

KW - ESI

KW - MALDI-TOF

KW - SELDI

KW - biomarkers

KW - classification and identification of bacteria

KW - cyanotoxins

KW - mass spectrometry

KW - polyphasic taxonomy

KW - proteomics

KW - BACTERIA

KW - STRAIN LEVEL

KW - TIME

KW - MALDI-TOF MS

KW - CYANOBACTERIA

KW - LASER-DESORPTION IONIZATION

KW - DESORPTION/IONISATION

KW - RAPID IDENTIFICATION

KW - ELECTROSPRAY-IONIZATION

KW - PROTEOMICS

UR - http://www.scopus.com/inward/record.url?scp=85073188504&partnerID=8YFLogxK

U2 - DOI: 10.1134/S0026261719050151

DO - DOI: 10.1134/S0026261719050151

M3 - Review article

VL - 88

SP - 534

EP - 547

JO - МИКРОБИОЛОГИЯ

JF - МИКРОБИОЛОГИЯ

SN - 0026-3656

IS - 5

ER -