TY - JOUR

T1 - cis-Golgi phosphate transporters harboring an EXS domain are essential for plant growth and development

AU - Hsieh, Yi-Fang

AU - Suslov, Dmitry

AU - Espen, Luca

AU - Schiavone, Marion

AU - Rautengarten, Carsten

AU - Griess-Osowski, Annika

AU - Voiniciuc, Catalin

AU - Poirier, Yves

N1 - Yi-Fang Hsieh, Dmitry Suslov, Luca Espen, Marion Schiavone, Carsten Rautengarten, Annika Griess-Osowski, Catalin Voiniciuc, Yves Poirier, cis-Golgi phosphate transporters harboring an EXS domain are essential for plant growth and development, Plant Physiology, 2023;, kiad123, https://doi.org/10.1093/plphys/kiad123

PY - 2023/5/31

Y1 - 2023/5/31

N2 - Cell wall synthesis and protein glycosylation require the import of nucleotide diphosphate–sugar conjugates into the Golgi that must be counterbalanced by phosphate (Pi) export. Numerous Golgi nucleotide-sugar transporters have been characterized, but transporters mediating Golgi Pi export remain poorly understood. We used plant and yeast genetics to characterize the role of 2 Arabidopsis (Arabidopsis thaliana) proteins possessing an EXS domain, namely ERD1A and ERD1B, in Golgi Pi homeostasis. ERD1A and ERD1B localized in cis-Golgi and were broadly expressed in vegetative and reproductive tissues. We identified ERD1 putative orthologs in algae, bryophytes, and vascular plants. Expressing ERD1A and ERD1B in yeast complemented the erd1 mutant phenotype of cellular Pi loss via exocytosis associated with reduced Golgi Pi export. The Arabidopsis erd1a mutant had a similar phenotype of apoplastic Pi loss dependent on exocytosis. ERD1A overexpression in Nicotiana benthamiana and Arabidopsis led to partial mislocalization of ERD1A to the plasma membrane and specific Pi export to the apoplastic space. Arabidopsis erd1a had defects in cell wall biosynthesis, which were associated with reduced shoot development, hypocotyl growth, cell wall extensibility, root elongation, pollen germination, pollen tube elongation, and fertility. We identified ERD1 proteins as Golgi Pi exporters that are essential for optimal plant growth and fertility.

AB - Cell wall synthesis and protein glycosylation require the import of nucleotide diphosphate–sugar conjugates into the Golgi that must be counterbalanced by phosphate (Pi) export. Numerous Golgi nucleotide-sugar transporters have been characterized, but transporters mediating Golgi Pi export remain poorly understood. We used plant and yeast genetics to characterize the role of 2 Arabidopsis (Arabidopsis thaliana) proteins possessing an EXS domain, namely ERD1A and ERD1B, in Golgi Pi homeostasis. ERD1A and ERD1B localized in cis-Golgi and were broadly expressed in vegetative and reproductive tissues. We identified ERD1 putative orthologs in algae, bryophytes, and vascular plants. Expressing ERD1A and ERD1B in yeast complemented the erd1 mutant phenotype of cellular Pi loss via exocytosis associated with reduced Golgi Pi export. The Arabidopsis erd1a mutant had a similar phenotype of apoplastic Pi loss dependent on exocytosis. ERD1A overexpression in Nicotiana benthamiana and Arabidopsis led to partial mislocalization of ERD1A to the plasma membrane and specific Pi export to the apoplastic space. Arabidopsis erd1a had defects in cell wall biosynthesis, which were associated with reduced shoot development, hypocotyl growth, cell wall extensibility, root elongation, pollen germination, pollen tube elongation, and fertility. We identified ERD1 proteins as Golgi Pi exporters that are essential for optimal plant growth and fertility.

UR - https://www.mendeley.com/catalogue/50744aff-7dbc-3e76-83f6-e8729c696118/

U2 - 10.1093/plphys/kiad123

DO - 10.1093/plphys/kiad123

M3 - Article

C2 - 36856724

VL - 192

SP - 1000

EP - 1015

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

M1 - kiad123

ER -