DOI

The damaging effect of ionizing radiation (IR) on skeletal muscle Na,K-ATPase is an open field of research. Considering a therapeutic potential of ouabain, a specific ligand of the Na,K-ATPase, we tested its ability to protect against the IR-induced disturbances of Na,K-ATPase function in rat diaphragm muscle that co-expresses the α1 and α2 isozymes of this protein. Male Wistar rats (n = 26) were subjected to 6-day injections of vehicle (0.9% NaCl) or ouabain (1 µg/kg/day). On the fourth day of injections, rats were exposed to one-time total-body X-ray irradiation (10 Gy), or a sham irradiation. The isolated muscles were studied 72 h post-irradiation. IR decreased the electrogenic contribution of the α2 Na,K-ATPase without affecting its protein content, thereby causing sarcolemma depolarization. IR increased serum concentrations of ouabain, IL-6, and corticosterone, decreased lipid peroxidation, and changed cellular redox status. Chronic ouabain administration prevented IR-induced depolarization and loss of the α2 Na,K-ATPase electrogenic contribution without changing its protein content. This was accompanied with an elevation of ouabain concentration in circulation and with the lack of IR-induced suppression of lipid peroxidation. Given the crucial role of Na,K-ATPase in skeletal muscle performance, these findings may have therapeutic implications as countermeasures for IR-induced muscle pathology.

Translated title of the contributionХронический уабаин предотвращает радиационно-индуцированное снижение функции α2 Na,K-АТФазы в диафрагмальной мышце крысы
Original languageEnglish
Article number10921
Number of pages19
JournalInternational Journal of Molecular Sciences
Volume23
Issue number18
DOIs
StatePublished - 18 Sep 2022

    Research areas

  • Na,K-ATPase isozymes, ionizing radiation, ouabain, resting membrane potential, skeletal muscle, Rats, Wistar, Rats, Male, Muscle, Skeletal/metabolism, Sodium/metabolism, Diaphragm/metabolism, Ouabain/metabolism, Animals, Saline Solution, Corticosterone/metabolism, Interleukin-6/metabolism, Sodium-Potassium-Exchanging ATPase/metabolism, Ligands, Isoenzymes/metabolism

    Scopus subject areas

  • Physiology
  • Molecular Biology
  • Spectroscopy
  • Catalysis
  • Inorganic Chemistry
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry

ID: 99758257