Chicken Microchromosomes in the Lampbrush Phase

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Chicken Microchromosomes in the Lampbrush Phase : A Cytogenetic Description. / Galkina, Svetlana; Fillon, Valerie; Saifitdinova, Alsu; Daks, Aleksandra; Kulak, Maria; Dyomin, Alexander; Koshel, Elena; Gaginskaya, Elena R.

In: Cytogenetic and Genome Research, Vol. 152, No. 1, 01.08.2017, p. 46-54.

Research output: Contribution to journal › Article › peer-review

Author

Galkina, Svetlana ; Fillon, Valerie ; Saifitdinova, Alsu ; Daks, Aleksandra ; Kulak, Maria ; Dyomin, Alexander ; Koshel, Elena ; Gaginskaya, Elena R. / Chicken Microchromosomes in the Lampbrush Phase : A Cytogenetic Description. In: Cytogenetic and Genome Research. 2017 ; Vol. 152, No. 1. pp. 46-54.

BibTeX

@article{edd854a4b9414dc089acce958ba46b6e,

title = "Chicken Microchromosomes in the Lampbrush Phase: A Cytogenetic Description",

abstract = "Lampbrush chromosomes are giant, transcriptionally active, meiotic chromosomes found in oocytes of all vertebrates with the exception of mammals. Lampbrush chromosomes offer a convenient tool for cytogenetic mapping and, in particular, have been instrumental in mapping genes and linkage groups on chicken (GGA) chromosomes. Whereas cytogenetic maps of macrochromosome GGA1-10 and microchromosome GGA11-16 lampbrush bivalents have been established, identification and description of smaller microchromosome bivalents are still missing. In this work, we used specific FISH probes for the identification of 12 chicken lampbrush chromosomes formed by GGA17-28. Our observations on chromomere and lateral loop arrangement and chiasma position allowed us to construct the respective cytogenetic maps for these microchromosomes. For the 10 smallest chicken microchromosomes, GGA29-38, no individual molecular tags are available, yet they can be collectively marked using the PO41 repeat. The reported results contribute to building of working cytogenetic maps of the chicken karyotype.",

keywords = "BAC clones, Cytogenetic maps, FISH mapping, Gallus gallus domesticus, Lampbrush chromosomes, Microchromosomes",

author = "Svetlana Galkina and Valerie Fillon and Alsu Saifitdinova and Aleksandra Daks and Maria Kulak and Alexander Dyomin and Elena Koshel and Gaginskaya, {Elena R.}",

note = "Publisher Copyright: {\textcopyright} 2017 S. Karger AG, Basel.",

year = "2017",

month = aug,

day = "1",

doi = "10.1159/000475563",

language = "English",

volume = "152",

pages = "46--54",

journal = "Cytogenetic and Genome Research",

issn = "1424-8581",

publisher = "S. Karger AG",

number = "1",

}

RIS

TY - JOUR

T1 - Chicken Microchromosomes in the Lampbrush Phase

T2 - A Cytogenetic Description

AU - Galkina, Svetlana

AU - Fillon, Valerie

AU - Saifitdinova, Alsu

AU - Daks, Aleksandra

AU - Kulak, Maria

AU - Dyomin, Alexander

AU - Koshel, Elena

AU - Gaginskaya, Elena R.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Lampbrush chromosomes are giant, transcriptionally active, meiotic chromosomes found in oocytes of all vertebrates with the exception of mammals. Lampbrush chromosomes offer a convenient tool for cytogenetic mapping and, in particular, have been instrumental in mapping genes and linkage groups on chicken (GGA) chromosomes. Whereas cytogenetic maps of macrochromosome GGA1-10 and microchromosome GGA11-16 lampbrush bivalents have been established, identification and description of smaller microchromosome bivalents are still missing. In this work, we used specific FISH probes for the identification of 12 chicken lampbrush chromosomes formed by GGA17-28. Our observations on chromomere and lateral loop arrangement and chiasma position allowed us to construct the respective cytogenetic maps for these microchromosomes. For the 10 smallest chicken microchromosomes, GGA29-38, no individual molecular tags are available, yet they can be collectively marked using the PO41 repeat. The reported results contribute to building of working cytogenetic maps of the chicken karyotype.

AB - Lampbrush chromosomes are giant, transcriptionally active, meiotic chromosomes found in oocytes of all vertebrates with the exception of mammals. Lampbrush chromosomes offer a convenient tool for cytogenetic mapping and, in particular, have been instrumental in mapping genes and linkage groups on chicken (GGA) chromosomes. Whereas cytogenetic maps of macrochromosome GGA1-10 and microchromosome GGA11-16 lampbrush bivalents have been established, identification and description of smaller microchromosome bivalents are still missing. In this work, we used specific FISH probes for the identification of 12 chicken lampbrush chromosomes formed by GGA17-28. Our observations on chromomere and lateral loop arrangement and chiasma position allowed us to construct the respective cytogenetic maps for these microchromosomes. For the 10 smallest chicken microchromosomes, GGA29-38, no individual molecular tags are available, yet they can be collectively marked using the PO41 repeat. The reported results contribute to building of working cytogenetic maps of the chicken karyotype.

KW - BAC clones

KW - Cytogenetic maps

KW - FISH mapping

KW - Gallus gallus domesticus

KW - Lampbrush chromosomes

KW - Microchromosomes

UR - http://www.scopus.com/inward/record.url?scp=85020217699&partnerID=8YFLogxK

U2 - 10.1159/000475563

DO - 10.1159/000475563

M3 - Article

C2 - 28564645

AN - SCOPUS:85020217699

VL - 152

SP - 46

EP - 54

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 1

ER -

ID: 97809391