Research output: Contribution to journal › Article › peer-review
Characterization of cytokine production by human term placenta macrophages in vitro. / Pavlov, Oleg; Pavlova, Oksana; Ailamazyan, Edouard; Selkov, Sergei.
In: American Journal of Reproductive Immunology, Vol. 60, No. 6, 2008, p. 556-567.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Characterization of cytokine production by human term placenta macrophages in vitro
AU - Pavlov, Oleg
AU - Pavlova, Oksana
AU - Ailamazyan, Edouard
AU - Selkov, Sergei
PY - 2008
Y1 - 2008
N2 - Problem: Macrophages are apparently the only immune cells within placenta villi, yet functions of these cells remain obscure. It has been postulated that placental macrophages accomplish regulatory roles at the fetal-maternal interface by means of wide variety of secreted cytokines. We attempt to analyze the patterns of cytokine production in an isolated population of placental macrophages. Method of study: Macrophages were obtained from term placentas in the absence of spontaneous labor. The basal and lipopolysaccharide (LPS)-stimulated levels of intracellular cytokines were detected by flow cytometry. The basal cytokine secretion was determined by BD™ Cytometry Bead Array (BD Biosciences, San Diego, CA, USA). Results: Intracellular IL-1α IL-1β, IL-6, and TNFα were detected in 31, 27, 4, and 3% CD68+ cells, respectively. Stimulation with LPS increased the proportions of cytokine-producing CD68+ cells to 48, 50, 28, and 49%, respectively. Under basal conditions, levels of released TNFα and IL-6, respectively, were 20- and 25-fold higher when compared with IL-1β while IL-10 was secreted in small but detectable amounts. When a secretory activity was estimated for cytokine-producing cells, the secretion rate for TNFα and IL-6 overwhelmingly surpassed that for IL-1β (TNFα:IL-6:IL-1β ratio was 192:145:1). Conclusion: These results suggest functional heterogeneity of the placental macrophage population and contribute to the elucidation of regulatory roles of these cells in gestation.
AB - Problem: Macrophages are apparently the only immune cells within placenta villi, yet functions of these cells remain obscure. It has been postulated that placental macrophages accomplish regulatory roles at the fetal-maternal interface by means of wide variety of secreted cytokines. We attempt to analyze the patterns of cytokine production in an isolated population of placental macrophages. Method of study: Macrophages were obtained from term placentas in the absence of spontaneous labor. The basal and lipopolysaccharide (LPS)-stimulated levels of intracellular cytokines were detected by flow cytometry. The basal cytokine secretion was determined by BD™ Cytometry Bead Array (BD Biosciences, San Diego, CA, USA). Results: Intracellular IL-1α IL-1β, IL-6, and TNFα were detected in 31, 27, 4, and 3% CD68+ cells, respectively. Stimulation with LPS increased the proportions of cytokine-producing CD68+ cells to 48, 50, 28, and 49%, respectively. Under basal conditions, levels of released TNFα and IL-6, respectively, were 20- and 25-fold higher when compared with IL-1β while IL-10 was secreted in small but detectable amounts. When a secretory activity was estimated for cytokine-producing cells, the secretion rate for TNFα and IL-6 overwhelmingly surpassed that for IL-1β (TNFα:IL-6:IL-1β ratio was 192:145:1). Conclusion: These results suggest functional heterogeneity of the placental macrophage population and contribute to the elucidation of regulatory roles of these cells in gestation.
KW - Cytokine secretion
KW - Intracellular cytokines
KW - Lipopolysaccharide
KW - Macrophages
KW - Term placenta
UR - http://www.scopus.com/inward/record.url?scp=56149120855&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0897.2008.00657.x
DO - 10.1111/j.1600-0897.2008.00657.x
M3 - Article
C2 - 18853988
AN - SCOPUS:56149120855
VL - 60
SP - 556
EP - 567
JO - Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy
JF - Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy
SN - 8755-8920
IS - 6
ER -
ID: 87787180