Research output: Contribution to journal › Article › peer-review
Cellulose synthesis and cell expansion are regulated by different mechanisms in growing arabidopsis hypocotyls. / Ivakov, Alexander; Flis, Anna; Apelt, Federico; Fünfgeld, Maximillian; Scherer, Ulrike; Stitt, Mark; Kragler, Friedrich; Vissenberg, Kris; Persson, Staffan; Suslov, Dmitry.
In: Plant Cell, Vol. 29, No. 6, 06.2017, p. 1305-1315.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Cellulose synthesis and cell expansion are regulated by different mechanisms in growing arabidopsis hypocotyls
AU - Ivakov, Alexander
AU - Flis, Anna
AU - Apelt, Federico
AU - Fünfgeld, Maximillian
AU - Scherer, Ulrike
AU - Stitt, Mark
AU - Kragler, Friedrich
AU - Vissenberg, Kris
AU - Persson, Staffan
AU - Suslov, Dmitry
N1 - Publisher Copyright: © 2017 ASPB.
PY - 2017/6
Y1 - 2017/6
N2 - Plant growth is sustained by two complementary processes: biomass biosynthesis and cell expansion. The cell wall is crucial to both as it forms the majority of biomass, while its extensibility limits cell expansion. Cellulose is a major component of the cell wall and cellulose synthesis is pivotal to plant cell growth, and its regulation is poorly understood. Using periodic diurnal variation in Arabidopsis thaliana hypocotyl growth, we found that cellulose synthesis and cell expansion can be uncoupled and are regulated by different mechanisms. We grew Arabidopsis plants in very short photoperiods and used a combination of extended nights, continuous light, sucrose feeding experiments, and photosynthesis inhibition to tease apart the influences of light, metabolic, and circadian clock signaling on rates of cellulose biosynthesis and cell wall biomechanics. We demonstrate that cell expansion is regulated by protein-mediated changes in cell wall extensibility driven by the circadian clock. By contrast, the biosynthesis of cellulose is controlled through intracellular trafficking of cellulose synthase enzyme complexes regulated exclusively by metabolic signaling related to the carbon status of the plant and independently of the circadian clock or light signaling.
AB - Plant growth is sustained by two complementary processes: biomass biosynthesis and cell expansion. The cell wall is crucial to both as it forms the majority of biomass, while its extensibility limits cell expansion. Cellulose is a major component of the cell wall and cellulose synthesis is pivotal to plant cell growth, and its regulation is poorly understood. Using periodic diurnal variation in Arabidopsis thaliana hypocotyl growth, we found that cellulose synthesis and cell expansion can be uncoupled and are regulated by different mechanisms. We grew Arabidopsis plants in very short photoperiods and used a combination of extended nights, continuous light, sucrose feeding experiments, and photosynthesis inhibition to tease apart the influences of light, metabolic, and circadian clock signaling on rates of cellulose biosynthesis and cell wall biomechanics. We demonstrate that cell expansion is regulated by protein-mediated changes in cell wall extensibility driven by the circadian clock. By contrast, the biosynthesis of cellulose is controlled through intracellular trafficking of cellulose synthase enzyme complexes regulated exclusively by metabolic signaling related to the carbon status of the plant and independently of the circadian clock or light signaling.
UR - http://www.scopus.com/inward/record.url?scp=85025687679&partnerID=8YFLogxK
U2 - 10.1105/tpc.16.00782
DO - 10.1105/tpc.16.00782
M3 - Article
C2 - 28550150
AN - SCOPUS:85025687679
VL - 29
SP - 1305
EP - 1315
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 6
ER -
ID: 97791105