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Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery. / Dubashynskaya, N.V.; Zhuravskii, S.G.; Borovskoy, A.Y.; Bokatyi, A.N.; Sall, T.S.; Egorova, T.S.; Demyanova, E.V.; Murashko, E.A.; Mukhametdinova, D.V.; Shasherina, A.Y.; Anufrikov, Y.A.; Skorik, Y.A.

In: Colloids Surf. A Physicochem. Eng. Asp., Vol. 726, 137788, 05.12.2025.

Research output: Contribution to journal › Article › peer-review

Harvard

Dubashynskaya, NV, Zhuravskii, SG, Borovskoy, AY, Bokatyi, AN, Sall, TS, Egorova, TS, Demyanova, EV, Murashko, EA, Mukhametdinova, DV, Shasherina, AY, Anufrikov, YA & Skorik, YA 2025, 'Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery', Colloids Surf. A Physicochem. Eng. Asp., vol. 726, 137788. https://doi.org/10.1016/j.colsurfa.2025.137788

APA

Dubashynskaya, N. V., Zhuravskii, S. G., Borovskoy, A. Y., Bokatyi, A. N., Sall, T. S., Egorova, T. S., Demyanova, E. V., Murashko, E. A., Mukhametdinova, D. V., Shasherina, A. Y., Anufrikov, Y. A., & Skorik, Y. A. (2025). Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery. Colloids Surf. A Physicochem. Eng. Asp., 726, [137788]. https://doi.org/10.1016/j.colsurfa.2025.137788

Vancouver

Dubashynskaya NV, Zhuravskii SG, Borovskoy AY, Bokatyi AN, Sall TS, Egorova TS et al. Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery. Colloids Surf. A Physicochem. Eng. Asp. 2025 Dec 5;726. 137788. https://doi.org/10.1016/j.colsurfa.2025.137788

Author

Dubashynskaya, N.V. ; Zhuravskii, S.G. ; Borovskoy, A.Y. ; Bokatyi, A.N. ; Sall, T.S. ; Egorova, T.S. ; Demyanova, E.V. ; Murashko, E.A. ; Mukhametdinova, D.V. ; Shasherina, A.Y. ; Anufrikov, Y.A. ; Skorik, Y.A. / Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery. In: Colloids Surf. A Physicochem. Eng. Asp. 2025 ; Vol. 726.

BibTeX

@article{54b30fb60ed846b1b54e231404a5de04,
title = "Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery",
abstract = "This study investigates the development of innovative polyelectrolyte complexes (PECs) based on polysaccharides, carrageenan (CRG) and cyanocobalamin-chitosan (CSB12), for the enhanced delivery of colistin (CT), a last-resort antibiotic used to treat multidrug-resistant Gram-negative bacterial infections. Two novel systems were engineered: CRG-CT and CRG-CT-CSB12 PECs. The CRG-CT PECs displayed a hydrodynamic diameter of 220 nm and a ζ-potential of −35.6 mV, whereas the CRG-CT-CSB12 PECs were smaller (140 nm) and exhibited a positive charge (ζ-potential of +30.1 mV). For CRG, the binding constants for CT interactions were reported for the first time, demonstrating a twofold increase in strength compared to those of hyaluronic acid. This enhanced binding affinity correlates with prolonged drug release and a more pronounced reduction in nephro- and neurotoxicity relative to previously established systems. Both systems demonstrated sustained release of CT, with only 23 % of the drug released over 24 h, and a two fold reduction in the minimum inhibitory concentration against Pseudomonas aeruginosa compared to free CT. Notably, the PECs showed no nephro- or neurotoxicity in human cell line models (HEK 293 and T-98G). The CRG-CT-CSB12 PECs also exhibited superior intestinal permeability (Papp = 7.4 × 10−6 cm/s) in Caco-2 cell monolayers, indicating their suitability for oral administration. In vivo studies in a rodent model confirmed the stability of CRG-CT PECs in the bloodstream and their lack of acute toxicity at therapeutic doses. These findings highlight the potential of CRG-CT and CRG-CT-CSB12 PECs as effective, and innovative nanoantibiotics for combating multidrug-resistant infections, with particular promise for oral delivery. {\textcopyright} 2025 Elsevier B.V., All rights reserved.",
keywords = "Carrageenan, Chitosan, Colistin, Cyanocobalamin, Intestinal permeability, Polyelectrolyte complexes, Antibiotics, Binding energy, Cell culture, Controlled drug delivery, Hyaluronic acid, Monolayers, Multilayers, Targeted drug delivery, Toxicity, Bacterial infections, Carrageenans, Hydrodynamic diameter, Intestinal permeabilities, Multidrug resistants, Nephrotoxicity, Neurotoxicity, carrageenan, chitosan, colistin, cyanocobalamin, hyaluronic acid, nanocarrier, polyelectrolyte, polysaccharide, acute toxicity, animal experiment, animal tissue, antibacterial activity, antimicrobial activity, Article, association constant, binding affinity, Caco-2 cell line, chemical structure, controlled study, cytotoxicity, drug blood level, drug delivery system, Gram negative infection, HEK293 cell line, human, human cell, hydrodynamics, in vivo study, intestine mucosa permeability, male, minimum inhibitory concentration, multidrug resistant Gram negative bacterium, nephrotoxicity, neurotoxicity, nonhuman, particle size, Pseudomonas aeruginosa, rat, rodent model, structure analysis, sustained drug release, T98G cell line, zeta potential",
author = "N.V. Dubashynskaya and S.G. Zhuravskii and A.Y. Borovskoy and A.N. Bokatyi and T.S. Sall and T.S. Egorova and E.V. Demyanova and E.A. Murashko and D.V. Mukhametdinova and A.Y. Shasherina and Y.A. Anufrikov and Y.A. Skorik",
note = "Export Date: 01 November 2025; Cited By: 0; Correspondence Address: Y.A. Skorik; Branch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds, St. Petersburg, Bolshoi VO 31, 199004, Russian Federation; email: yury_skorik@mail.ru; CODEN: CPEAE",
year = "2025",
month = dec,
day = "5",
doi = "10.1016/j.colsurfa.2025.137788",
language = "Английский",
volume = "726",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
issn = "0927-7757",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Carrageenan and cyanocobalamin-chitosan based nanoantibiotics: An effective strategy for colistin delivery

AU - Dubashynskaya, N.V.

AU - Zhuravskii, S.G.

AU - Borovskoy, A.Y.

AU - Bokatyi, A.N.

AU - Sall, T.S.

AU - Egorova, T.S.

AU - Demyanova, E.V.

AU - Murashko, E.A.

AU - Mukhametdinova, D.V.

AU - Shasherina, A.Y.

AU - Anufrikov, Y.A.

AU - Skorik, Y.A.

N1 - Export Date: 01 November 2025; Cited By: 0; Correspondence Address: Y.A. Skorik; Branch of Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds, St. Petersburg, Bolshoi VO 31, 199004, Russian Federation; email: yury_skorik@mail.ru; CODEN: CPEAE

PY - 2025/12/5

Y1 - 2025/12/5

N2 - This study investigates the development of innovative polyelectrolyte complexes (PECs) based on polysaccharides, carrageenan (CRG) and cyanocobalamin-chitosan (CSB12), for the enhanced delivery of colistin (CT), a last-resort antibiotic used to treat multidrug-resistant Gram-negative bacterial infections. Two novel systems were engineered: CRG-CT and CRG-CT-CSB12 PECs. The CRG-CT PECs displayed a hydrodynamic diameter of 220 nm and a ζ-potential of −35.6 mV, whereas the CRG-CT-CSB12 PECs were smaller (140 nm) and exhibited a positive charge (ζ-potential of +30.1 mV). For CRG, the binding constants for CT interactions were reported for the first time, demonstrating a twofold increase in strength compared to those of hyaluronic acid. This enhanced binding affinity correlates with prolonged drug release and a more pronounced reduction in nephro- and neurotoxicity relative to previously established systems. Both systems demonstrated sustained release of CT, with only 23 % of the drug released over 24 h, and a two fold reduction in the minimum inhibitory concentration against Pseudomonas aeruginosa compared to free CT. Notably, the PECs showed no nephro- or neurotoxicity in human cell line models (HEK 293 and T-98G). The CRG-CT-CSB12 PECs also exhibited superior intestinal permeability (Papp = 7.4 × 10−6 cm/s) in Caco-2 cell monolayers, indicating their suitability for oral administration. In vivo studies in a rodent model confirmed the stability of CRG-CT PECs in the bloodstream and their lack of acute toxicity at therapeutic doses. These findings highlight the potential of CRG-CT and CRG-CT-CSB12 PECs as effective, and innovative nanoantibiotics for combating multidrug-resistant infections, with particular promise for oral delivery. © 2025 Elsevier B.V., All rights reserved.

AB - This study investigates the development of innovative polyelectrolyte complexes (PECs) based on polysaccharides, carrageenan (CRG) and cyanocobalamin-chitosan (CSB12), for the enhanced delivery of colistin (CT), a last-resort antibiotic used to treat multidrug-resistant Gram-negative bacterial infections. Two novel systems were engineered: CRG-CT and CRG-CT-CSB12 PECs. The CRG-CT PECs displayed a hydrodynamic diameter of 220 nm and a ζ-potential of −35.6 mV, whereas the CRG-CT-CSB12 PECs were smaller (140 nm) and exhibited a positive charge (ζ-potential of +30.1 mV). For CRG, the binding constants for CT interactions were reported for the first time, demonstrating a twofold increase in strength compared to those of hyaluronic acid. This enhanced binding affinity correlates with prolonged drug release and a more pronounced reduction in nephro- and neurotoxicity relative to previously established systems. Both systems demonstrated sustained release of CT, with only 23 % of the drug released over 24 h, and a two fold reduction in the minimum inhibitory concentration against Pseudomonas aeruginosa compared to free CT. Notably, the PECs showed no nephro- or neurotoxicity in human cell line models (HEK 293 and T-98G). The CRG-CT-CSB12 PECs also exhibited superior intestinal permeability (Papp = 7.4 × 10−6 cm/s) in Caco-2 cell monolayers, indicating their suitability for oral administration. In vivo studies in a rodent model confirmed the stability of CRG-CT PECs in the bloodstream and their lack of acute toxicity at therapeutic doses. These findings highlight the potential of CRG-CT and CRG-CT-CSB12 PECs as effective, and innovative nanoantibiotics for combating multidrug-resistant infections, with particular promise for oral delivery. © 2025 Elsevier B.V., All rights reserved.

KW - Carrageenan

KW - Chitosan

KW - Colistin

KW - Cyanocobalamin

KW - Intestinal permeability

KW - Polyelectrolyte complexes

KW - Antibiotics

KW - Binding energy

KW - Cell culture

KW - Controlled drug delivery

KW - Hyaluronic acid

KW - Monolayers

KW - Multilayers

KW - Targeted drug delivery

KW - Toxicity

KW - Bacterial infections

KW - Carrageenans

KW - Hydrodynamic diameter

KW - Intestinal permeabilities

KW - Multidrug resistants

KW - Nephrotoxicity

KW - Neurotoxicity

KW - carrageenan

KW - chitosan

KW - colistin

KW - cyanocobalamin

KW - hyaluronic acid

KW - nanocarrier

KW - polyelectrolyte

KW - polysaccharide

KW - acute toxicity

KW - animal experiment

KW - animal tissue

KW - antibacterial activity

KW - antimicrobial activity

KW - Article

KW - association constant

KW - binding affinity

KW - Caco-2 cell line

KW - chemical structure

KW - controlled study

KW - cytotoxicity

KW - drug blood level

KW - drug delivery system

KW - Gram negative infection

KW - HEK293 cell line

KW - human

KW - human cell

KW - hydrodynamics

KW - in vivo study

KW - intestine mucosa permeability

KW - male

KW - minimum inhibitory concentration

KW - multidrug resistant Gram negative bacterium

KW - nephrotoxicity

KW - neurotoxicity

KW - nonhuman

KW - particle size

KW - Pseudomonas aeruginosa

KW - rat

KW - rodent model

KW - structure analysis

KW - sustained drug release

KW - T98G cell line

KW - zeta potential

UR - https://www.mendeley.com/catalogue/4656747b-3397-3d49-b006-92f781a00025/

U2 - 10.1016/j.colsurfa.2025.137788

DO - 10.1016/j.colsurfa.2025.137788

M3 - статья

VL - 726

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - 137788

ER -

ID: 143195033