For the first time in the Russian Federation, a study was conducted to determine the level of mercury in the hair of young and middle-aged women with or without metabolic syndrome (MS) and the Association of mercury content in hair with cardiometabolic risk factors. The studied cohort consisted of 387 women (204 with metabolic syndrome and 183 without metabolic syndrome as controls). The mercury content in the hair was determined by atomic absorption pyrolysis. The relationship between mercury in hair and cardiometabolic risk factors was evaluated using linear regression. Calculation of the odds ratio (OR) of the risk of metabolic syndrome among quartiles of mercury content in the hair was performed by multiple logistic regression. Hg concentrations in hair were higher in women with metabolic syndrome than without metabolic syndrome (Mean – 520; median – 360; versus Mean – 330; median – 260 µg/kg, P < 0.001). There is an increased risk of developing cardiometabolic syndrome in women, whose hair contain over 544 µg/kg of mercury. The results of this study indicate that the intake of Hg in the human body can be one of the factors that can increase the development of cardiometabolic risk.