Research output: Contribution to journal › Article › peer-review
Carbon-mixed dental cement for fixing fiber optic ferrules prevents visually triggered locomotive enhancement in mice upon optogenetic stimulation. / Araragi, Naozumi; Alenina, Natalia; Bader, Michael.
In: Heliyon, Vol. 8, No. 1, e08692, 01.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Carbon-mixed dental cement for fixing fiber optic ferrules prevents visually triggered locomotive enhancement in mice upon optogenetic stimulation
AU - Araragi, Naozumi
AU - Alenina, Natalia
AU - Bader, Michael
N1 - Publisher Copyright: © 2021 The Author(s)
PY - 2022/1
Y1 - 2022/1
N2 - Optogenetics enables activation/silencing of specific neurons with unprecedented temporal and spatial resolution. The method, however, is prone to artefacts associated with biophysics of light used for illuminating opsin-expressing neurons. Here we employed Tph2-mhChR2-YFP transgenic mice, which express channelrhodopsin (ChR2) only in serotonergic neurons in the brain, to investigate behavioral effects of optogenetic stimulation of serotonergic neurons. Surprisingly, optogenetic stimulation enhanced locomotion even in ChR2-negative mice. Such unspecific effects are likely to be due to visual agitation caused by light leakage from the dental cement, which is commonly used to fixate optic fiber ferrules on the skull. When we employed black dental cement made by mixing carbons with dental cement powders, such unspecific effects were abolished in ChR2-negative mice, but not in ChR2-positive mice, confirming that enhanced locomotion resulted from serotonergic activation. The method allows extracting genuine behavioral effects of optogenetic stimulation without contamination from visual stimuli caused by light leakage.
AB - Optogenetics enables activation/silencing of specific neurons with unprecedented temporal and spatial resolution. The method, however, is prone to artefacts associated with biophysics of light used for illuminating opsin-expressing neurons. Here we employed Tph2-mhChR2-YFP transgenic mice, which express channelrhodopsin (ChR2) only in serotonergic neurons in the brain, to investigate behavioral effects of optogenetic stimulation of serotonergic neurons. Surprisingly, optogenetic stimulation enhanced locomotion even in ChR2-negative mice. Such unspecific effects are likely to be due to visual agitation caused by light leakage from the dental cement, which is commonly used to fixate optic fiber ferrules on the skull. When we employed black dental cement made by mixing carbons with dental cement powders, such unspecific effects were abolished in ChR2-negative mice, but not in ChR2-positive mice, confirming that enhanced locomotion resulted from serotonergic activation. The method allows extracting genuine behavioral effects of optogenetic stimulation without contamination from visual stimuli caused by light leakage.
KW - Carbon
KW - Dental cement
KW - Light leakage
KW - Locomotion
KW - Serotonin (5-HT)
UR - http://www.scopus.com/inward/record.url?scp=85121973656&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/6deeef90-5491-391c-a15b-94fe93e8228a/
U2 - 10.1016/j.heliyon.2021.e08692
DO - 10.1016/j.heliyon.2021.e08692
M3 - Article
AN - SCOPUS:85121973656
VL - 8
JO - Heliyon
JF - Heliyon
SN - 2405-8440
IS - 1
M1 - e08692
ER -
ID: 100672496