TY - JOUR

T1 - Bringing new methods to the seed proteomics platform

T2 - Challenges and perspectives

AU - Smolikova, Galina

AU - Gorbach, Daria

AU - Lukasheva, Elena

AU - Mavropolo‐stolyarenko, Gregory

AU - Bilova, Tatiana

AU - Soboleva, Alena

AU - Tsarev, Alexander

AU - Romanovskaya, Ekaterina

AU - Podolskaya, Ekaterina

AU - Zhukov, Vladimir

AU - Tikhonovich, Igor

AU - Medvedev, Sergei

AU - Hoehenwarter, Wolfgang

AU - Frolov, Andrej

N1 - Smolikova, G.; Gorbach, D.; Lukasheva, E.; Mavropolo-Stolyarenko, G.; Bilova, T.; Soboleva, A.; Tsarev, A.; Romanovskaya, E.; Podolskaya, E.; Zhukov, V.; Tikhonovich, I.; Medvedev, S.; Hoehenwarter, W.; Frolov, A. Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives. Int. J. Mol. Sci. 2020, 21, 9162.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins, and carbohydrates in their seeds, distinctly dominate modern agriculture, thus play an essential role in food industry and fuel production. Therefore, seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, seed development and germination as well as age‐ and stress‐related alterations in seed vigor, longevity, nutritional value, and safety can be addressed by a broad panel of analytical, biochemical, and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes accompanying plant development, senescence, and response to biotic or abiotic stress. Among individual post‐genomic methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. During the recent decades, multiple methodological advances were introduced in different branches of life science, although only some of them were established in seed proteomics so far. Therefore, here we discuss main methodological approaches already employed in seed proteomics, as well as those still waiting for implementation in this field of plant research, with a special emphasis on sample preparation, data acquisition, processing, and post‐processing. Thereby, the overall goal of this review is to bring new methodologies emerging in different areas of proteomics research (clinical, food, ecological, microbial, and plant proteomics) to the broad society of seed biologists.

AB - For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins, and carbohydrates in their seeds, distinctly dominate modern agriculture, thus play an essential role in food industry and fuel production. Therefore, seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, seed development and germination as well as age‐ and stress‐related alterations in seed vigor, longevity, nutritional value, and safety can be addressed by a broad panel of analytical, biochemical, and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes accompanying plant development, senescence, and response to biotic or abiotic stress. Among individual post‐genomic methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. During the recent decades, multiple methodological advances were introduced in different branches of life science, although only some of them were established in seed proteomics so far. Therefore, here we discuss main methodological approaches already employed in seed proteomics, as well as those still waiting for implementation in this field of plant research, with a special emphasis on sample preparation, data acquisition, processing, and post‐processing. Thereby, the overall goal of this review is to bring new methodologies emerging in different areas of proteomics research (clinical, food, ecological, microbial, and plant proteomics) to the broad society of seed biologists.

KW - Data processing

KW - Gel‐based proteomics

KW - Gel‐free proteomics

KW - Glycation

KW - Glycosylation

KW - Phosphorylation

KW - Post‐translational modifications

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=85097121396&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/b24cc03f-ba84-3ba5-8cff-eaaa75582ef9/

U2 - 10.3390/ijms21239162

DO - 10.3390/ijms21239162

M3 - Review article

C2 - 33271881

AN - SCOPUS:85097121396

VL - 21

SP - 1

EP - 54

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 23

M1 - 9162

ER -