Research output: Contribution to journal › Article › peer-review
Bovine gamma-interferon nuclear localization sequence provides translocation of recombinant protein to yeast Pichia pastoris cell nucleus. / Gradoboeva, A.E.; Padkina, M.V.
In: Cell and Tissue Biology, Vol. 4, No. 6, 2010, p. 566-571.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Bovine gamma-interferon nuclear localization sequence provides translocation of recombinant protein to yeast Pichia pastoris cell nucleus
AU - Gradoboeva, A.E.
AU - Padkina, M.V.
PY - 2010
Y1 - 2010
N2 - It is known that the expression of heterologous protein production in microorganisms has a negative influence on the host cell. Therefore, to utilize microorganisms for production of recombinant proteins it is necessary the follow the fate of recombinant proteins in cells. In this study, we constructed a modified bovine IFNG gene that encodes interferon with ten amino-acid deletions at the C-terminal. We also generated genetic constructs that ensured the expression of native and modified bovine IFNG fused with GFP gene in yeast Pichia pastoris. The expression of IFN-γ/GFP and IFN-γ(Δ10)/GFP chimeric proteins showed that bovine IFN-γ nuclear localization signal was functioned in yeast cells. The absence of these proteins leads to the cytoplasmic accumulation of recombinant protein. © 2010 Pleiades Publishing, Ltd.
AB - It is known that the expression of heterologous protein production in microorganisms has a negative influence on the host cell. Therefore, to utilize microorganisms for production of recombinant proteins it is necessary the follow the fate of recombinant proteins in cells. In this study, we constructed a modified bovine IFNG gene that encodes interferon with ten amino-acid deletions at the C-terminal. We also generated genetic constructs that ensured the expression of native and modified bovine IFNG fused with GFP gene in yeast Pichia pastoris. The expression of IFN-γ/GFP and IFN-γ(Δ10)/GFP chimeric proteins showed that bovine IFN-γ nuclear localization signal was functioned in yeast cells. The absence of these proteins leads to the cytoplasmic accumulation of recombinant protein. © 2010 Pleiades Publishing, Ltd.
U2 - 10.1134/S1990519X10060076
DO - 10.1134/S1990519X10060076
M3 - Article
VL - 4
SP - 566
EP - 571
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 6
ER -
ID: 8183003