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Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging. / Критченков, Илья Сергеевич; Соломатина, Анастасия Игоревна; Козина, Дарья Олеговна; Порсев, Виталий Вениаминович; Соколов, Виктор Владимирович; Ширманова, Марина; Лукина, Мария; Комарова, Анастасия Денисовна; Щеславский, Владислав; Беляева, Татьяна; Литвинов, Илья; Салова, Анна; Корнилова, Елена Сергеевна; Качкин, Даниил Валерьевич; Туник, Сергей Павлович.

In: Molecules, Vol. 26, No. 10, 2898, 13.05.2021.

Research output: Contribution to journal › Article › peer-review

Harvard

Критченков, ИС, Соломатина, АИ, Козина, ДО, Порсев, ВВ, Соколов, ВВ, Ширманова, М, Лукина, М, Комарова, АД, Щеславский, В, Беляева, Т, Литвинов, И, Салова, А, Корнилова, ЕС, Качкин, ДВ & Туник, СП 2021, 'Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging', Molecules, vol. 26, no. 10, 2898. https://doi.org/10.3390/molecules26102898

APA

Критченков, И. С., Соломатина, А. И., Козина, Д. О., Порсев, В. В., Соколов, В. В., Ширманова, М., Лукина, М., Комарова, А. Д., Щеславский, В., Беляева, Т., Литвинов, И., Салова, А., Корнилова, Е. С., Качкин, Д. В., & Туник, С. П. (2021). Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging. Molecules, 26(10), [2898]. https://doi.org/10.3390/molecules26102898

Vancouver

Критченков ИС, Соломатина АИ, Козина ДО, Порсев ВВ, Соколов ВВ, Ширманова М et al. Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging. Molecules. 2021 May 13;26(10). 2898. https://doi.org/10.3390/molecules26102898

Author

Критченков, Илья Сергеевич ; Соломатина, Анастасия Игоревна ; Козина, Дарья Олеговна ; Порсев, Виталий Вениаминович ; Соколов, Виктор Владимирович ; Ширманова, Марина ; Лукина, Мария ; Комарова, Анастасия Денисовна ; Щеславский, Владислав ; Беляева, Татьяна ; Литвинов, Илья ; Салова, Анна ; Корнилова, Елена Сергеевна ; Качкин, Даниил Валерьевич ; Туник, Сергей Павлович. / Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging. In: Molecules. 2021 ; Vol. 26, No. 10.

BibTeX

@article{cf2e366ada8045d18b34d6d1abafc82e,
title = "Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging",
abstract = "Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir(NˆC) 2 (NˆN)] + complexes (NˆC–benzothienyl-phenanthridine based cyclometalated ligand; NˆN–pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters{\textquoteright} solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological “window of transparency”, NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0–2.3 µs under normoxia and 2.8–3.0 µs under hypoxia conditions) in complete agreement with the calibration curves obtained “in cuvette”. The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems. ",
keywords = "Ir(III) complexes, NIR emitters, Oxygen sensing, Phosphorescence lifetime imaging, Biocompatible Materials/chemistry, Cricetulus, Humans, Molecular Conformation, Iridium/chemistry, Luminescence, Subcellular Fractions/metabolism, Animals, Proton Magnetic Resonance Spectroscopy, Oxygen/analysis, HeLa Cells, CHO Cells, MITOCHONDRIA, oxygen sensing, CYCLOMETALATED PLATINUM(II), phosphorescence lifetime imaging, EMISSIVE BEHAVIOR, LUMINESCENT RHENIUM(I), CELLS, PHOTOPHYSICAL PROPERTIES, PROBES, NANOPARTICLES, IRIDIUM(III) POLYPYRIDINE COMPLEXES, IN-VIVO",
author = "Критченков, {Илья Сергеевич} and Соломатина, {Анастасия Игоревна} and Козина, {Дарья Олеговна} and Порсев, {Виталий Вениаминович} and Соколов, {Виктор Владимирович} and Марина Ширманова and Мария Лукина and Комарова, {Анастасия Денисовна} and Владислав Щеславский and Татьяна Беляева and Илья Литвинов and Анна Салова and Корнилова, {Елена Сергеевна} and Качкин, {Даниил Валерьевич} and Туник, {Сергей Павлович}",
note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = may,
day = "13",
doi = "10.3390/molecules26102898",
language = "English",
volume = "26",
journal = "Molecules",
issn = "1420-3049",
publisher = "MDPI AG",
number = "10",

}

RIS

TY - JOUR

T1 - Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging

AU - Критченков, Илья Сергеевич

AU - Соломатина, Анастасия Игоревна

AU - Козина, Дарья Олеговна

AU - Порсев, Виталий Вениаминович

AU - Соколов, Виктор Владимирович

AU - Ширманова, Марина

AU - Лукина, Мария

AU - Комарова, Анастасия Денисовна

AU - Щеславский, Владислав

AU - Беляева, Татьяна

AU - Литвинов, Илья

AU - Салова, Анна

AU - Корнилова, Елена Сергеевна

AU - Качкин, Даниил Валерьевич

AU - Туник, Сергей Павлович

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/5/13

Y1 - 2021/5/13

N2 - Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir(NˆC) 2 (NˆN)] + complexes (NˆC–benzothienyl-phenanthridine based cyclometalated ligand; NˆN–pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters’ solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological “window of transparency”, NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0–2.3 µs under normoxia and 2.8–3.0 µs under hypoxia conditions) in complete agreement with the calibration curves obtained “in cuvette”. The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems.

AB - Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir(NˆC) 2 (NˆN)] + complexes (NˆC–benzothienyl-phenanthridine based cyclometalated ligand; NˆN–pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters’ solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological “window of transparency”, NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0–2.3 µs under normoxia and 2.8–3.0 µs under hypoxia conditions) in complete agreement with the calibration curves obtained “in cuvette”. The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems.

KW - Ir(III) complexes

KW - NIR emitters

KW - Oxygen sensing

KW - Phosphorescence lifetime imaging

KW - Biocompatible Materials/chemistry

KW - Cricetulus

KW - Humans

KW - Molecular Conformation

KW - Iridium/chemistry

KW - Luminescence

KW - Subcellular Fractions/metabolism

KW - Animals

KW - Proton Magnetic Resonance Spectroscopy

KW - Oxygen/analysis

KW - HeLa Cells

KW - CHO Cells

KW - MITOCHONDRIA

KW - oxygen sensing

KW - CYCLOMETALATED PLATINUM(II)

KW - phosphorescence lifetime imaging

KW - EMISSIVE BEHAVIOR

KW - LUMINESCENT RHENIUM(I)

KW - CELLS

KW - PHOTOPHYSICAL PROPERTIES

KW - PROBES

KW - NANOPARTICLES

KW - IRIDIUM(III) POLYPYRIDINE COMPLEXES

KW - IN-VIVO

UR - http://www.scopus.com/inward/record.url?scp=85106589070&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/04bc84d5-0a60-3585-86e4-3829fc506741/

U2 - 10.3390/molecules26102898

DO - 10.3390/molecules26102898

M3 - Article

C2 - 34068190

VL - 26

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 10

M1 - 2898

ER -

ID: 76792612