Research output: Contribution to journal › Article › peer-review
Benchmarking state-of-the-art approaches for norovirus genome assembly in metagenome sample. / Мелешко, Дмитрий Алексеевич; Коробейников, Антон Иванович.
In: Biology, Vol. 12, No. 8, 1066, 29.07.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Benchmarking state-of-the-art approaches for norovirus genome assembly in metagenome sample
AU - Мелешко, Дмитрий Алексеевич
AU - Коробейников, Антон Иванович
PY - 2023/7/29
Y1 - 2023/7/29
N2 - A recently published article in BMCGenomics by Fuentes-Trillo et al. contains a comparison of assembly approaches of several noroviral samples via different tools and preprocessing strategies. It turned out that the study used outdated versions of tools as well as tools that were not designed for the viral assembly task. In order to improve the suboptimal assemblies, authors suggested different sophisticated preprocessing strategies that seem to make only minor contributions to the results. We have reproduced the analysis using state-of-the-art tools designed for viral assembly, and we demonstrate that tools from the SPAdes toolkit (rnaviralSPAdes and coronaSPAdes) allow one to assemble the samples from the original study into a single contig without any additional preprocessing.
AB - A recently published article in BMCGenomics by Fuentes-Trillo et al. contains a comparison of assembly approaches of several noroviral samples via different tools and preprocessing strategies. It turned out that the study used outdated versions of tools as well as tools that were not designed for the viral assembly task. In order to improve the suboptimal assemblies, authors suggested different sophisticated preprocessing strategies that seem to make only minor contributions to the results. We have reproduced the analysis using state-of-the-art tools designed for viral assembly, and we demonstrate that tools from the SPAdes toolkit (rnaviralSPAdes and coronaSPAdes) allow one to assemble the samples from the original study into a single contig without any additional preprocessing.
KW - benchmarking
KW - noroviruses
KW - viral assembly
UR - https://www.mendeley.com/catalogue/c35913cd-0715-3518-becd-133500c0e148/
U2 - 10.3390/biology12081066
DO - 10.3390/biology12081066
M3 - Article
C2 - 37626951
VL - 12
JO - Biology
JF - Biology
SN - 2079-7737
IS - 8
M1 - 1066
ER -
ID: 106999471