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Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species. / Zlatogursky, Vasily V.; Klimov, Vladimir I.

In: Protist, Vol. 167, 2016, p. 555-567.

Research output: Contribution to journal › Article

Harvard

Zlatogursky, VV & Klimov, VI 2016, 'Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species', Protist, vol. 167, pp. 555-567. https://doi.org/10.1016/j.protis.2016.09.004

APA

Zlatogursky, V. V., & Klimov, V. I. (2016). Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species. Protist, 167, 555-567. https://doi.org/10.1016/j.protis.2016.09.004

Vancouver

Zlatogursky VV, Klimov VI. Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species. Protist. 2016;167:555-567. https://doi.org/10.1016/j.protis.2016.09.004

Author

Zlatogursky, Vasily V. ; Klimov, Vladimir I. / Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species. In: Protist. 2016 ; Vol. 167. pp. 555-567.

BibTeX

@article{523a02049c6a47c08b9a211ee99115e3,
title = "Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species",
abstract = "The first application of DNA-barcoding for the centrohelids is reported. The character-rich genus Acanthocystis was chosen to compare sequence divergence and morphological similarity. Acanthocystis nichollsi, an easily identifiable and well outlined species, was isolated from four remote locations; A. costata; A. takahashii (2 strains) and A. turfacea were studied as well. Detailed light- and electron-microscopic data were obtained and a fragment of 18S rDNA (mostly V5 to V8 regions) was cloned and multiple clones were sequenced. Obtained data allowed a comparison of the level of genetic divergence between several strains of one and the same morphospecies from remote locations and several strains of different morphospecies. This analysis showed no overlap between intraspecific and interspecific divergence. Phylogenetic analysis also recovered the clades for all species correctly. The genetic divergence between individual molecular clones obtained from the same clonal culture allowed accessing the genetic stru",
keywords = "Centrohelids, identification, phylogeny, DNA-barcoding, 18S rDNA",
author = "Zlatogursky, {Vasily V.} and Klimov, {Vladimir I.}",
year = "2016",
doi = "10.1016/j.protis.2016.09.004",
language = "English",
volume = "167",
pages = "555--567",
journal = "Protist",
issn = "1434-4610",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Barcoding Heliozoa: Perspectives of 18S rDNA for Distinguishing Between Acanthocystis Species

AU - Zlatogursky, Vasily V.

AU - Klimov, Vladimir I.

PY - 2016

Y1 - 2016

N2 - The first application of DNA-barcoding for the centrohelids is reported. The character-rich genus Acanthocystis was chosen to compare sequence divergence and morphological similarity. Acanthocystis nichollsi, an easily identifiable and well outlined species, was isolated from four remote locations; A. costata; A. takahashii (2 strains) and A. turfacea were studied as well. Detailed light- and electron-microscopic data were obtained and a fragment of 18S rDNA (mostly V5 to V8 regions) was cloned and multiple clones were sequenced. Obtained data allowed a comparison of the level of genetic divergence between several strains of one and the same morphospecies from remote locations and several strains of different morphospecies. This analysis showed no overlap between intraspecific and interspecific divergence. Phylogenetic analysis also recovered the clades for all species correctly. The genetic divergence between individual molecular clones obtained from the same clonal culture allowed accessing the genetic stru

AB - The first application of DNA-barcoding for the centrohelids is reported. The character-rich genus Acanthocystis was chosen to compare sequence divergence and morphological similarity. Acanthocystis nichollsi, an easily identifiable and well outlined species, was isolated from four remote locations; A. costata; A. takahashii (2 strains) and A. turfacea were studied as well. Detailed light- and electron-microscopic data were obtained and a fragment of 18S rDNA (mostly V5 to V8 regions) was cloned and multiple clones were sequenced. Obtained data allowed a comparison of the level of genetic divergence between several strains of one and the same morphospecies from remote locations and several strains of different morphospecies. This analysis showed no overlap between intraspecific and interspecific divergence. Phylogenetic analysis also recovered the clades for all species correctly. The genetic divergence between individual molecular clones obtained from the same clonal culture allowed accessing the genetic stru

KW - Centrohelids

KW - identification

KW - phylogeny

KW - DNA-barcoding

KW - 18S rDNA

U2 - 10.1016/j.protis.2016.09.004

DO - 10.1016/j.protis.2016.09.004

M3 - Article

VL - 167

SP - 555

EP - 567

JO - Protist

JF - Protist

SN - 1434-4610

ER -

ID: 7598068