To visualize phytohormone localization in plant tissues, transgenic plants comprising the GUS reporter gene are often used. However, until now only qualitative assessment of the hormone presence was available. In this work, we suggested the method for IAA quantification in transgenic DR5::GUS Arabidopsis thaliana L. plants by the analysis of digital images. An empirical quadratic dependence was established between the IAA concentration in medium and the level of GUS-dependent staining. Using this method, we demonstrated that, after A. thaliana root gravistimulation for 90 min, auxin lateral redistribution occurred. It resulted in the increase in the IAA concentration in the lower root part (in the elongation zone and apical meristem) by 200% on the average.

Original languageEnglish
Pages (from-to)706-711
Number of pages6
JournalRussian Journal of Plant Physiology
Volume55
Issue number5
DOIs
StatePublished - 1 Sep 2008

    Research areas

  • Arabidopsis thaliana, Digital image color analysis, Gravitropism, GUS reporter gene, Histochemical staining, IAA

    Scopus subject areas

  • Plant Science

ID: 36021923