Research output: Contribution to journal › Article › peer-review
AuNP Aptasensor for Hodgkin Lymphoma Monitoring. / Slyusarenko, Maria; Shalaev, Sergey; Valitova, Alina; Zabegina, Lidia; Nikiforova, Nadezhda; Nazarova, Inga; Rudakovskaya, Polina; Vorobiev, Maxim; Lezov, Alexey; Filatova, Larisa; Yevlampieva, Natalia; Gorin, Dmitry; Krzhivitsky, Pavel; Malek, Anastasia.
In: Biosensors, Vol. 12, No. 1, 23, 04.01.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - AuNP Aptasensor for Hodgkin Lymphoma Monitoring
AU - Slyusarenko, Maria
AU - Shalaev, Sergey
AU - Valitova, Alina
AU - Zabegina, Lidia
AU - Nikiforova, Nadezhda
AU - Nazarova, Inga
AU - Rudakovskaya, Polina
AU - Vorobiev, Maxim
AU - Lezov, Alexey
AU - Filatova, Larisa
AU - Yevlampieva, Natalia
AU - Gorin, Dmitry
AU - Krzhivitsky, Pavel
AU - Malek, Anastasia
N1 - Funding Information: Funding: This research was supported by the Ministry of Health of the Russian Federation (project no. AAAA-A18-118032890186-5). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/1/4
Y1 - 2022/1/4
N2 - A liquid biopsy based on circulating small extracellular vesicles (SEVs) has not yet been used in routine clinical practice due to the lack of reliable analytic technologies. Recent studies have demonstrated the great diagnostic potential of nanozyme-based systems for the detection of SEV markers. Here, we hypothesize that CD30-positive Hodgkin and Reed–Sternberg (HRS) cells secrete CD30 + SEVs; therefore, the relative amount of circulating CD30 + SEVs might reflect classical forms of Hodgkin lymphoma (cHL) activity and can be measured by using a nanozyme-based technique. A AuNP aptasensor analytics system was created using aurum nanoparticles (AuNPs) with peroxidase activity. Sensing was mediated by competing properties of DNA aptamers to attach onto surface of AuNPs inhibiting their enzymatic activity and to bind specific markers on SEVs surface. An enzymatic activity of AuNPs was evaluated through the color reaction. The study included characterization of the components of the analytic system and its functionality using transmission and scanning electron microscopy, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), and spectrophotometry. AuNP aptasensor analytics were optimized to quantify plasma CD30 + SEVs. The developed method allowed us to differentiate healthy donors and cHL patients. The results of the CD30 + SEV quantification in the plasma of cHL patients were compared with the results of disease activity assessment by positron emission tomography/computed tomography (PET-CT) scanning, revealing a strong positive correlation. Moreover, two cycles of chemotherapy resulted in a statistically significant decrease in CD30 + SEVs in the plasma of cHL patients. The proposed AuNP aptasensor system presents a promising new approach for monitoring cHL patients and can be modified for the diagnostic testing of other diseases.
AB - A liquid biopsy based on circulating small extracellular vesicles (SEVs) has not yet been used in routine clinical practice due to the lack of reliable analytic technologies. Recent studies have demonstrated the great diagnostic potential of nanozyme-based systems for the detection of SEV markers. Here, we hypothesize that CD30-positive Hodgkin and Reed–Sternberg (HRS) cells secrete CD30 + SEVs; therefore, the relative amount of circulating CD30 + SEVs might reflect classical forms of Hodgkin lymphoma (cHL) activity and can be measured by using a nanozyme-based technique. A AuNP aptasensor analytics system was created using aurum nanoparticles (AuNPs) with peroxidase activity. Sensing was mediated by competing properties of DNA aptamers to attach onto surface of AuNPs inhibiting their enzymatic activity and to bind specific markers on SEVs surface. An enzymatic activity of AuNPs was evaluated through the color reaction. The study included characterization of the components of the analytic system and its functionality using transmission and scanning electron microscopy, nanoparticle tracking analysis (NTA), dynamic light scattering (DLS), and spectrophotometry. AuNP aptasensor analytics were optimized to quantify plasma CD30 + SEVs. The developed method allowed us to differentiate healthy donors and cHL patients. The results of the CD30 + SEV quantification in the plasma of cHL patients were compared with the results of disease activity assessment by positron emission tomography/computed tomography (PET-CT) scanning, revealing a strong positive correlation. Moreover, two cycles of chemotherapy resulted in a statistically significant decrease in CD30 + SEVs in the plasma of cHL patients. The proposed AuNP aptasensor system presents a promising new approach for monitoring cHL patients and can be modified for the diagnostic testing of other diseases.
KW - Aptamer
KW - Diagnostic
KW - Enzyme-mimetic nanoparticles
KW - Gold nanoparticles
KW - Hodgkin lymphoma
KW - Liquid biopsy
KW - Monitoring
KW - Small extracellular vesicles
KW - gold nanoparticles
KW - monitoring
KW - enzyme-mimetic nanoparticles
KW - ELECTROCHEMICAL DETECTION
KW - EXTRACELLULAR VESICLES
KW - aptamer
KW - small extracellular vesicles
KW - diagnostic
KW - liquid biopsy
KW - PEROXIDASE-LIKE ACTIVITY
UR - http://www.scopus.com/inward/record.url?scp=85123101423&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/64817dfc-6523-303f-ac14-7ee0638df1f1/
U2 - 10.3390/bios12010023
DO - 10.3390/bios12010023
M3 - Article
C2 - 35049651
AN - SCOPUS:85123101423
VL - 12
JO - Biosensors
JF - Biosensors
SN - 2079-6374
IS - 1
M1 - 23
ER -
ID: 93143134