TY - JOUR

T1 - Association Study of Symbiotic Genes in Pea (Pisum sativum L.) Cultivars Grown in Symbiotic Conditions

AU - Zhukov , Vladimir A.

AU - Zhernakov, Aleksandr I.

AU - Sulima, Anton S.

AU - Кулаева, Ольга Алексеевна

AU - Клюкова, Марина Сергеевна

AU - Afonin, Alexey M.

AU - Shtark , Oksana Yu.

AU - Тихонович, Игорь Анатольевич

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11

Y1 - 2021/11

N2 - In garden pea (Pisum sativum L.), several symbiotic genes are known to control the development of mutualistic symbioses with nodule bacteria (NB) and arbuscular mycorrhizal fungi (AMF). Here, we studied whether the allelic state of the symbiotic genes was associated with the growth parameters of pea plants under single inoculation with NB and under double inoculation with NB + AMF. Using different statistical methods, we analyzed the dataset obtained from a pot experiment that involved 99 pea cultivars, 10 of which were characterized as having shortened intern-odes due to the presence of the natural mutation p.A229T in the developmental gene Le. The plant’s habitus strongly influenced most of the studied growth and yield parameters and the effectiveness of the symbiotic interactions under NB and NB + AMF inoculation. Double inoculation had different effects on Le + (normal) and le − (dwarf) plants with regard to nitrogen and phosphorus content in seeds. Regardless of the Le-status of plants, allelic states of the symbiotic gene LykX encoding the putative receptor of Nod factors (bacterial signal molecules) were shown to be associated with seed number, thousand-seed weight, and pod number at the level of FDR < 0.001, whereas associations of allelic states of the other studied symbiotic genes were less significant.

AB - In garden pea (Pisum sativum L.), several symbiotic genes are known to control the development of mutualistic symbioses with nodule bacteria (NB) and arbuscular mycorrhizal fungi (AMF). Here, we studied whether the allelic state of the symbiotic genes was associated with the growth parameters of pea plants under single inoculation with NB and under double inoculation with NB + AMF. Using different statistical methods, we analyzed the dataset obtained from a pot experiment that involved 99 pea cultivars, 10 of which were characterized as having shortened intern-odes due to the presence of the natural mutation p.A229T in the developmental gene Le. The plant’s habitus strongly influenced most of the studied growth and yield parameters and the effectiveness of the symbiotic interactions under NB and NB + AMF inoculation. Double inoculation had different effects on Le + (normal) and le − (dwarf) plants with regard to nitrogen and phosphorus content in seeds. Regardless of the Le-status of plants, allelic states of the symbiotic gene LykX encoding the putative receptor of Nod factors (bacterial signal molecules) were shown to be associated with seed number, thousand-seed weight, and pod number at the level of FDR < 0.001, whereas associations of allelic states of the other studied symbiotic genes were less significant.

KW - Arbuscular mycorrhiza

KW - Nodule bacteria

KW - Pisum sativum

KW - Symbiotic effectiveness

KW - Symbiotic genes

KW - Symbiotic responsivity

KW - PHOSPHORUS

KW - ARBUSCULAR MYCORRHIZA

KW - nodule bacteria

KW - NODULATION

KW - NITROGEN-FIXATION

KW - QUANTITATIVE TRAIT LOCI

KW - symbiotic effectiveness

KW - symbiotic genes

KW - ROOT COLONIZATION

KW - symbiotic responsivity

KW - EXPRESSION

KW - arbuscular mycorrhiza

UR - http://www.scopus.com/inward/record.url?scp=85120084868&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/9033a4d2-aabc-396f-b74a-581fba15629f/

U2 - 10.3390/agronomy11112368

DO - 10.3390/agronomy11112368

M3 - Article

VL - 11

JO - Agronomy

JF - Agronomy

SN - 2073-4395

IS - 11

M1 - 2368

ER -