Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis. / Yudintceva, Natalia M.; Bogolyubova, Irina O.; Muraviov, Alexandr N.; Sheykhov, Magomed G.; Vinogradova, Tatiana I. ; Sokolovich, Evgenii G.; Samusenko, Igor A.; Shevtsov, Maxim A.
In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 3, 01.03.2018, p. e1580-e1593.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Application of the allogenic mesenchymal stem cells in the therapy of the bladder tuberculosis
AU - Yudintceva, Natalia M.
AU - Bogolyubova, Irina O.
AU - Muraviov, Alexandr N.
AU - Sheykhov, Magomed G.
AU - Vinogradova, Tatiana I.
AU - Sokolovich, Evgenii G.
AU - Samusenko, Igor A.
AU - Shevtsov, Maxim A.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.
AB - Urogenital tuberculosis (TB) often leads to contraction of the bladder, a reduction of the urinary reservoir capacity, and, in the latest stage, to real microcystitis up to full obliteration. Bladder TB Stage 4 is unsuitable for conservative therapy, and cystectomy with subsequent enteroplasty is indicated. In this study, using a model of bladder TB in New Zealand rabbits, the therapeutic efficacy of the interstitial injection of autologous bone-derived mesenchymal stem cells (MSCs) combined with standard anti-TB treatment in the restoration of the bladder function was demonstrated. For analysis of the MSC distribution in tissues, the latter were labelled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated the high intracellular incorporation of nanoparticles and the absence of cytotoxicity on MSC viability and proliferation. A single-dose administration of MSCs into the bladder mucosal layer significantly reduced the wall deformation and inflammation and hindered the development of fibrosis, which was proven by the subsequent histological assay. Confocal microscopy studies of the bladder cryosections confirmed the presence of superparamagnetic iron oxide nanoparticle-labelled MSCs in different bladder layers of the treated animals, thus indicating the role of stem cells in bladder regeneration.
KW - bladder
KW - fibrosis
KW - mesenchymal stem cells
KW - Mycobacterium tuberculosis
KW - superparamagnetic iron oxide nanoparticles
KW - urogenital tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=85043390640&partnerID=8YFLogxK
U2 - 10.1002/term.2583
DO - 10.1002/term.2583
M3 - Article
AN - SCOPUS:85043390640
VL - 12
SP - e1580-e1593
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 3
ER -
ID: 36354458