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Application of spectral methods for studying of DNA damage induced by gamma-radiation. / Tankovskaia, Svetlana A. ; Kotb, Omar M. ; Dommes, Olga A. ; Paston, Sofia V. .

In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 200, 2018, p. 85-92.

Research output: Contribution to journal › Conference article

Harvard

Tankovskaia, SA, Kotb, OM, Dommes, OA & Paston, SV 2018, 'Application of spectral methods for studying of DNA damage induced by gamma-radiation', Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 200, pp. 85-92.

APA

Tankovskaia, S. A., Kotb, O. M., Dommes, O. A., & Paston, S. V. (2018). Application of spectral methods for studying of DNA damage induced by gamma-radiation. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 200, 85-92.

Vancouver

Tankovskaia SA, Kotb OM, Dommes OA, Paston SV. Application of spectral methods for studying of DNA damage induced by gamma-radiation. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2018;200:85-92.

Author

Tankovskaia, Svetlana A. ; Kotb, Omar M. ; Dommes, Olga A. ; Paston, Sofia V. . / Application of spectral methods for studying of DNA damage induced by gamma-radiation. In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2018 ; Vol. 200. pp. 85-92.

BibTeX

@article{1f03116997d74e0ab2c822829bb05413,
title = "Application of spectral methods for studying of DNA damage induced by gamma-radiation",
abstract = "Spectral methods can provide a variety of possibilities to determine several types of radiation-induced DNA damage, such as nucleobase destruction and local denaturation. DNA UV absorption and CD spectra measured at room temperature undergo noticeable alteration under the action of γ-radiation. We have applied the Spirin method of total nucleobases determination, and have measured the molar extinction coefficient of DNA and DNA CD spectra for solutions with different NaCl concentrations (3 mM–3.2 M) and containing MgCl2, exposed to γ-radiation with the doses of 0–103 Gy. The melting temperatures of DNA in irradiated solutions at the doses of 0–50 Gy were obtained with the help of spectrophotometric melting. It was found that the amount of destructed nucleobases and radiation-induced loss of DNA helicity significantly decreases with the rise of the ionic strength of the irradiated solution. Substitution of a portion of Na+ ions on Mg2+ while keeping the total ionic strength constant (μ = 5 mM) does not affect the considered radiation effects. The role of the structure and composition of the DNA secondary hydration layer in the radiation-induced damages is discussed.",
author = "Tankovskaia, {Svetlana A.} and Kotb, {Omar M.} and Dommes, {Olga A.} and Paston, {Sofia V.}",
year = "2018",
language = "English",
volume = "200",
pages = "85--92",
journal = "SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY",
issn = "1386-1425",
publisher = "Elsevier",
note = "XIVh International Conference on Molecular Spectroscopy ; Conference date: 03-09-2017 Through 07-09-2017",

}

RIS

TY - JOUR

T1 - Application of spectral methods for studying of DNA damage induced by gamma-radiation

AU - Tankovskaia, Svetlana A.

AU - Kotb, Omar M.

AU - Dommes, Olga A.

AU - Paston, Sofia V.

PY - 2018

Y1 - 2018

N2 - Spectral methods can provide a variety of possibilities to determine several types of radiation-induced DNA damage, such as nucleobase destruction and local denaturation. DNA UV absorption and CD spectra measured at room temperature undergo noticeable alteration under the action of γ-radiation. We have applied the Spirin method of total nucleobases determination, and have measured the molar extinction coefficient of DNA and DNA CD spectra for solutions with different NaCl concentrations (3 mM–3.2 M) and containing MgCl2, exposed to γ-radiation with the doses of 0–103 Gy. The melting temperatures of DNA in irradiated solutions at the doses of 0–50 Gy were obtained with the help of spectrophotometric melting. It was found that the amount of destructed nucleobases and radiation-induced loss of DNA helicity significantly decreases with the rise of the ionic strength of the irradiated solution. Substitution of a portion of Na+ ions on Mg2+ while keeping the total ionic strength constant (μ = 5 mM) does not affect the considered radiation effects. The role of the structure and composition of the DNA secondary hydration layer in the radiation-induced damages is discussed.

AB - Spectral methods can provide a variety of possibilities to determine several types of radiation-induced DNA damage, such as nucleobase destruction and local denaturation. DNA UV absorption and CD spectra measured at room temperature undergo noticeable alteration under the action of γ-radiation. We have applied the Spirin method of total nucleobases determination, and have measured the molar extinction coefficient of DNA and DNA CD spectra for solutions with different NaCl concentrations (3 mM–3.2 M) and containing MgCl2, exposed to γ-radiation with the doses of 0–103 Gy. The melting temperatures of DNA in irradiated solutions at the doses of 0–50 Gy were obtained with the help of spectrophotometric melting. It was found that the amount of destructed nucleobases and radiation-induced loss of DNA helicity significantly decreases with the rise of the ionic strength of the irradiated solution. Substitution of a portion of Na+ ions on Mg2+ while keeping the total ionic strength constant (μ = 5 mM) does not affect the considered radiation effects. The role of the structure and composition of the DNA secondary hydration layer in the radiation-induced damages is discussed.

UR - https://proxy.library.spbu.ru:2068/science/article/pii/S138614251830310X#!

M3 - Conference article

VL - 200

SP - 85

EP - 92

JO - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

JF - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

SN - 1386-1425

T2 - XIVh International Conference on Molecular Spectroscopy

Y2 - 3 September 2017 through 7 September 2017

ER -

ID: 97645669