Research output: Contribution to journal › Article › peer-review
Application of spectral methods for studying DNA damage induced by gamma-radiation. / Tankovskaia, Svetlana A.; Kotb, Omar M.; Dommes, Olga A.; Paston, Sofia V.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 200, 05.07.2018, p. 85-92.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Application of spectral methods for studying DNA damage induced by gamma-radiation
AU - Tankovskaia, Svetlana A.
AU - Kotb, Omar M.
AU - Dommes, Olga A.
AU - Paston, Sofia V.
PY - 2018/7/5
Y1 - 2018/7/5
N2 - Spectral methods can provide a variety of possibilities to determine several types of radiation-induced DNA damage, such as nucleobase destruction and local denaturation. DNA UV absorption and CD spectra measured at room temperature undergo noticeable alteration under the action of γ-radiation. We have applied the Spirin method of total nucleobases determination, and have measured the molar extinction coefficient of DNA and DNA CD spectra for solutions with different NaCl concentrations (3 mM–3.2 M) and containing MgCl 2, exposed to γ-radiation with the doses of 0–10 3 Gy. The melting temperatures of DNA in irradiated solutions at the doses of 0–50 Gy were obtained with the help of spectrophotometric melting. It was found that the amount of destructed nucleobases and radiation-induced loss of DNA helicity significantly decreases with the rise of the ionic strength of the irradiated solution. Substitution of a portion of Na + ions on Mg 2+ while keeping the total ionic strength constant (μ = 5 mM) does not affect the considered radiation effects. The role of the structure and composition of the DNA secondary hydration layer in the radiation-induced damages is discussed.
AB - Spectral methods can provide a variety of possibilities to determine several types of radiation-induced DNA damage, such as nucleobase destruction and local denaturation. DNA UV absorption and CD spectra measured at room temperature undergo noticeable alteration under the action of γ-radiation. We have applied the Spirin method of total nucleobases determination, and have measured the molar extinction coefficient of DNA and DNA CD spectra for solutions with different NaCl concentrations (3 mM–3.2 M) and containing MgCl 2, exposed to γ-radiation with the doses of 0–10 3 Gy. The melting temperatures of DNA in irradiated solutions at the doses of 0–50 Gy were obtained with the help of spectrophotometric melting. It was found that the amount of destructed nucleobases and radiation-induced loss of DNA helicity significantly decreases with the rise of the ionic strength of the irradiated solution. Substitution of a portion of Na + ions on Mg 2+ while keeping the total ionic strength constant (μ = 5 mM) does not affect the considered radiation effects. The role of the structure and composition of the DNA secondary hydration layer in the radiation-induced damages is discussed.
KW - Circular dichroism
KW - DNA damage
KW - DNA hydration
KW - DNA melting
KW - Ionizing radiation
KW - Ions
KW - Nucleobases destruction
KW - UV absorption spectroscopy
KW - HYDRATED DNA
KW - NUCLEIC-ACID
KW - RAMAN-SPECTROSCOPY
KW - CIRCULAR-DICHROISM
KW - CONFORMATIONAL-CHANGES
KW - CELLULAR-DNA
KW - PLASMID DNA
KW - IONIC-STRENGTH
KW - METAL-IONS
KW - Nudeobases destruction
KW - REAL-TIME
UR - http://www.scopus.com/inward/record.url?scp=85045392890&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2018.04.011
DO - 10.1016/j.saa.2018.04.011
M3 - Article
VL - 200
SP - 85
EP - 92
JO - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
JF - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
SN - 1386-1425
ER -
ID: 19002242