Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

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Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae. / Ефремова, Елена Павловна ; Землянко, Ольга Михайловна ; Журавлева, Галина Анатольевна.

In: Microbiology, Vol. 93, No. 2, 02.04.2024, p. 236-239.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{8cab275e979d422c944cb1e6da1ff108,

title = "Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae",

abstract = "Abstract : Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.",

keywords = "SUP35, SUP45, Saccharomyces cerevisiae, flow cytometry, nonsense mutations, viability",

author = "Ефремова, {Елена Павловна} and Землянко, {Ольга Михайловна} and Журавлева, {Галина Анатольевна}",

note = "Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.",

year = "2024",

month = apr,

day = "2",

doi = "10.1134/s0026261723604244",

language = "English",

volume = "93",

pages = "236--239",

journal = "МИКРОБИОЛОГИЯ",

issn = "0026-3656",

publisher = "Издательство {"}Наука{"}",

number = "2",

}

RIS

TY - JOUR

T1 - Application of Flow Cytometry for Viability Assay of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

AU - Ефремова, Елена Павловна

AU - Землянко, Ольга Михайловна

AU - Журавлева, Галина Анатольевна

N1 - Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

PY - 2024/4/2

Y1 - 2024/4/2

N2 - Abstract : Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

AB - Abstract : Nonsense mutations in the essential SUP45 and SUP35 genes encoding translation termination factors affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of the mutants was 3.5‒4 times lower compared to the wild-type strains. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

KW - SUP35

KW - SUP45

KW - Saccharomyces cerevisiae

KW - flow cytometry

KW - nonsense mutations

KW - viability

UR - https://www.mendeley.com/catalogue/9441254e-c0f5-3d67-b419-f1dcce98d98c/

U2 - 10.1134/s0026261723604244

DO - 10.1134/s0026261723604244

M3 - Article

VL - 93

SP - 236

EP - 239

JO - МИКРОБИОЛОГИЯ

JF - МИКРОБИОЛОГИЯ

SN - 0026-3656

IS - 2

ER -

ID: 118906770