Application of Celestine Blue B and Gallocyanine for Studying the Effect of Drugs on the Production of Reactive Oxygen and Halogen Species by Neutrophils

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Application of Celestine Blue B and Gallocyanine for Studying the Effect of Drugs on the Production of Reactive Oxygen and Halogen Species by Neutrophils. / Reut, V. E.; Grigorieva, D. V.; Gorudko, I. V.; Sokolov, A. V.; Panasenko, O. M.

In: Journal of Applied Spectroscopy, Vol. 87, No. 4, 01.09.2020, p. 693-700.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{4c3194799ea44e6c8d6d73f6e09eaf84,

title = "Application of Celestine Blue B and Gallocyanine for Studying the Effect of Drugs on the Production of Reactive Oxygen and Halogen Species by Neutrophils",

abstract = "We investigated the effect of drugs (dapsone, paracetamol, and isoniazid) on the production of reactive oxygen and halogen species by neutrophils. The standard fluorescent method using scopoletin as well as recently developed fluorescent methods using oxazine dyes, celestine blue B, and gallocyanine were employed for this purpose. Celestine blue B reacts selectively with hypochlorous acid, while gallocyanine reacts mainly with the superoxide radical-anion, which reveals the regulatory effect of anti-inflammatory drugs on neutrophil NADPH-oxidase and myeloperoxidase responsible for the production of reactive oxygen and halogen species, respectively. These results indicate that gallocyanine and celestine blue B hold promise as chemosensors for studying the effect of drugs used in anti-inflammatory therapy for the neutrophil respiratory burst.",

keywords = "dapsone, isoniazid, neutrophil activation, oxidative stress, paracetamol, reactive halogen species, reactive oxygen species",

author = "Reut, {V. E.} and Grigorieva, {D. V.} and Gorudko, {I. V.} and Sokolov, {A. V.} and Panasenko, {O. M.}",

year = "2020",

month = sep,

day = "1",

doi = "10.1007/s10812-020-01056-5",

language = "English",

volume = "87",

pages = "693--700",

journal = "Journal of Applied Spectroscopy (English Translation of Zhurnal Prikladnoi Spektroskopii)",

issn = "0021-9037",

publisher = "Springer Nature",

number = "4",

}

RIS

TY - JOUR

T1 - Application of Celestine Blue B and Gallocyanine for Studying the Effect of Drugs on the Production of Reactive Oxygen and Halogen Species by Neutrophils

AU - Reut, V. E.

AU - Grigorieva, D. V.

AU - Gorudko, I. V.

AU - Sokolov, A. V.

AU - Panasenko, O. M.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - We investigated the effect of drugs (dapsone, paracetamol, and isoniazid) on the production of reactive oxygen and halogen species by neutrophils. The standard fluorescent method using scopoletin as well as recently developed fluorescent methods using oxazine dyes, celestine blue B, and gallocyanine were employed for this purpose. Celestine blue B reacts selectively with hypochlorous acid, while gallocyanine reacts mainly with the superoxide radical-anion, which reveals the regulatory effect of anti-inflammatory drugs on neutrophil NADPH-oxidase and myeloperoxidase responsible for the production of reactive oxygen and halogen species, respectively. These results indicate that gallocyanine and celestine blue B hold promise as chemosensors for studying the effect of drugs used in anti-inflammatory therapy for the neutrophil respiratory burst.

AB - We investigated the effect of drugs (dapsone, paracetamol, and isoniazid) on the production of reactive oxygen and halogen species by neutrophils. The standard fluorescent method using scopoletin as well as recently developed fluorescent methods using oxazine dyes, celestine blue B, and gallocyanine were employed for this purpose. Celestine blue B reacts selectively with hypochlorous acid, while gallocyanine reacts mainly with the superoxide radical-anion, which reveals the regulatory effect of anti-inflammatory drugs on neutrophil NADPH-oxidase and myeloperoxidase responsible for the production of reactive oxygen and halogen species, respectively. These results indicate that gallocyanine and celestine blue B hold promise as chemosensors for studying the effect of drugs used in anti-inflammatory therapy for the neutrophil respiratory burst.

KW - dapsone

KW - isoniazid

KW - neutrophil activation

KW - oxidative stress

KW - paracetamol

KW - reactive halogen species

KW - reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=85090797372&partnerID=8YFLogxK

U2 - 10.1007/s10812-020-01056-5

DO - 10.1007/s10812-020-01056-5

M3 - Article

AN - SCOPUS:85090797372

VL - 87

SP - 693

EP - 700

JO - Journal of Applied Spectroscopy (English Translation of Zhurnal Prikladnoi Spektroskopii)

JF - Journal of Applied Spectroscopy (English Translation of Zhurnal Prikladnoi Spektroskopii)

SN - 0021-9037

IS - 4

ER -

ID: 75024313