Research output: Contribution to journal › Article › peer-review
AP1-подобные цис-элементы в 5'-регуляторной области гена аполипопротеина A-I человека. / Lapikov, I. A.; Mogilenko, D. A.; Dizhe, E. B.; Ignatovich, I. A.; Orlov, S. V.; Perevozchikov, A. P.
In: МОЛЕКУЛЯРНАЯ БИОЛОГИЯ, Vol. 42, No. 2, 03.2008, p. 295-305.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - AP1-подобные цис-элементы в 5'-регуляторной области гена аполипопротеина A-I человека
AU - Lapikov, I. A.
AU - Mogilenko, D. A.
AU - Dizhe, E. B.
AU - Ignatovich, I. A.
AU - Orlov, S. V.
AU - Perevozchikov, A. P.
PY - 2008/3
Y1 - 2008/3
N2 - Several Ap1-like cis-acting elements were found within 5'-regulatory region (-2497...+173 versus transcription start point) of human apolipoprotein A-I gene (5'-apoA-I). Those elements are capable to interact with transcription factors belonging to Ap1 and CREB/ATF families. Those elements are localized outside of the hepatic enhancer (-220...-110) and the sequence responsible for apoA-I gene transcription in Caco2 cells (-595...-192). One of Ap1-like sites (5'-TGAGGTCT-3, Cre/jun2/apo) is present within 5'-apoA-I in two copies - distal (-1798 ...-1791) and proximal (+99...+106) ones. This and other Ap1-like sites - 5'-TGACTCT-3' (-1798...-1791, PF1) and 5'-TGACATCA-3' (-1171...-1163, Cre/jun1) were characterized by EMSA. It was shown by using the specific antibodies to c-Jun and ATF2 transcription factors in EMSA supershift experiments, that the DNA-protein complexes formed by Cre/jun2/apo, Cre/jun1 elements with nuclear proteins of human hepatoma HepG2 cells contain ATF2. The functional role of 5'-apoA-I regions containing Ap1-like sites was studied in cotransfection experiments of HepG2 cells (synthesize endogenous ApoA-I), human duodenum adenocarcinoma Hutu80 cells (do not synthesize endogenous ApoA-I), human neuroblastoma SK-N-SH cells (do not synthesize endogenous A-I) with expression vectors of c-jun and mekk1 genes. It was shown, that those Ap1-like sites appears to be responsible (the proximal Cre/jun2/apo is more efficient) for tissue-specific regulation of human apoA-I gene expression.
AB - Several Ap1-like cis-acting elements were found within 5'-regulatory region (-2497...+173 versus transcription start point) of human apolipoprotein A-I gene (5'-apoA-I). Those elements are capable to interact with transcription factors belonging to Ap1 and CREB/ATF families. Those elements are localized outside of the hepatic enhancer (-220...-110) and the sequence responsible for apoA-I gene transcription in Caco2 cells (-595...-192). One of Ap1-like sites (5'-TGAGGTCT-3, Cre/jun2/apo) is present within 5'-apoA-I in two copies - distal (-1798 ...-1791) and proximal (+99...+106) ones. This and other Ap1-like sites - 5'-TGACTCT-3' (-1798...-1791, PF1) and 5'-TGACATCA-3' (-1171...-1163, Cre/jun1) were characterized by EMSA. It was shown by using the specific antibodies to c-Jun and ATF2 transcription factors in EMSA supershift experiments, that the DNA-protein complexes formed by Cre/jun2/apo, Cre/jun1 elements with nuclear proteins of human hepatoma HepG2 cells contain ATF2. The functional role of 5'-apoA-I regions containing Ap1-like sites was studied in cotransfection experiments of HepG2 cells (synthesize endogenous ApoA-I), human duodenum adenocarcinoma Hutu80 cells (do not synthesize endogenous ApoA-I), human neuroblastoma SK-N-SH cells (do not synthesize endogenous A-I) with expression vectors of c-jun and mekk1 genes. It was shown, that those Ap1-like sites appears to be responsible (the proximal Cre/jun2/apo is more efficient) for tissue-specific regulation of human apoA-I gene expression.
UR - http://www.scopus.com/inward/record.url?scp=48049097054&partnerID=8YFLogxK
M3 - статья
C2 - 18610838
AN - SCOPUS:48049097054
VL - 42
SP - 295
EP - 305
JO - МОЛЕКУЛЯРНАЯ БИОЛОГИЯ
JF - МОЛЕКУЛЯРНАЯ БИОЛОГИЯ
SN - 0026-8984
IS - 2
ER -
ID: 91966248