TY - JOUR

T1 - ANTIOXIDATIVE PROTECTION AND MONOXIGENASE SYSTEM ACTIVITY OF CYTOCHROME P450 (MOG) OF NEUTROPHYLIC LEUKOCYTES (NL) FROM SYNOVIAL FLUID (SF) IN RHEUMATOID ARTHRITIS (RA)

AU - Shishkin, Viktor

AU - Kudriavtseva, Galina

AU - Malenkov, Yuri

AU - Shishkin, Viktor V.

PY - 2019/6

Y1 - 2019/6

N2 - Background: MOG system is present not only in the liver but also in far less quantities in other organs and tissues, including SF.Objectives: SF in RA patient contains up to 95% of NL that determines the inflammation also by inhibiting MOG.Methods: SF of knee joints of 8 patients with RA with active synovitis and 4 donors (D) were examined. NL have been isolated by standard methods. After the application of Triton-X-100 0,1% solution, NL has been centrifuged at 25000 g for 30 minutes. The supernatant has been used in the experiment. The activity of MOG has been estimated according to the level of cytochromes P450. P420 and b5 have been estimated by applying the method of differential spectrophotometry. Active forms of oxygen have been registered by applying electron paramagnetic resonance (EPR). The activity of the antioxidant enzymes - Cu-Zn superoxiddismutase (Cu-Zn SOD), Se-glutathione peroxidase (Se-GPO) and catalase (CAT) was estimated by employing classical enzymology methods.Results: It has been found out that in RA, the level of active oxygen forms increases by 68%. Meanwhile, the enzymes level of antioxidant defense is insufficient. In RA the activity of SOD, GPO and CAT enzymes is inhibited at 52, 66 and 39% respectively. In RA the activity of MOG (P450 and b5) in NL is 4 times lower than normal. The concentration of the inactive form of cytochrome P420, however, doubles. Nevertheless, their ratio P450/P420 (when normal = 5) in case of RA = 5,6 hardly ever changes.

AB - Background: MOG system is present not only in the liver but also in far less quantities in other organs and tissues, including SF.Objectives: SF in RA patient contains up to 95% of NL that determines the inflammation also by inhibiting MOG.Methods: SF of knee joints of 8 patients with RA with active synovitis and 4 donors (D) were examined. NL have been isolated by standard methods. After the application of Triton-X-100 0,1% solution, NL has been centrifuged at 25000 g for 30 minutes. The supernatant has been used in the experiment. The activity of MOG has been estimated according to the level of cytochromes P450. P420 and b5 have been estimated by applying the method of differential spectrophotometry. Active forms of oxygen have been registered by applying electron paramagnetic resonance (EPR). The activity of the antioxidant enzymes - Cu-Zn superoxiddismutase (Cu-Zn SOD), Se-glutathione peroxidase (Se-GPO) and catalase (CAT) was estimated by employing classical enzymology methods.Results: It has been found out that in RA, the level of active oxygen forms increases by 68%. Meanwhile, the enzymes level of antioxidant defense is insufficient. In RA the activity of SOD, GPO and CAT enzymes is inhibited at 52, 66 and 39% respectively. In RA the activity of MOG (P450 and b5) in NL is 4 times lower than normal. The concentration of the inactive form of cytochrome P420, however, doubles. Nevertheless, their ratio P450/P420 (when normal = 5) in case of RA = 5,6 hardly ever changes.

UR - https://ard.bmj.com/content/78/Suppl_2/1524.1.citation-tools

U2 - 10.1136/annrheumdis-2019-eular.836

DO - 10.1136/annrheumdis-2019-eular.836

M3 - Meeting Abstract

VL - 78

SP - 1524

EP - 1524

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - S2

M1 - AB0128

T2 - Annual European Congress of Rheumatology (EULAR 2019)

Y2 - 12 June 2019 through 15 June 2019

ER -