Anti-Influenza Activity of Enterocin B In vitro and Protective Effect of Bacteriocinogenic Enterococcal Probiotic Strain on Influenza Infection in Mouse Model.

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Anti-Influenza Activity of Enterocin B In vitro and Protective Effect of Bacteriocinogenic Enterococcal Probiotic Strain on Influenza Infection in Mouse Model. / Ermolenko, E. I.; Desheva, Y. A.; Kolobov, A. A.; Kotyleva, M. P.; Sychev, I. A.; Suvorov, A. N.

In: Probiotics and Antimicrobial Proteins, Vol. 11, No. 2, 2018, p. 705-712.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{8e7926c8395543c5b5a27ebc9a048e7c,

title = "Anti-Influenza Activity of Enterocin B In vitro and Protective Effect of Bacteriocinogenic Enterococcal Probiotic Strain on Influenza Infection in Mouse Model.",

abstract = "In spite of scientific evidence demonstrating the antiviral activity of lactic-acids bacteria, little is known about the mechanism of their action. Previously, several bacteriocins isolated from lactic acid bacteria (LAB) and some other microorganisms were reported as having antiviral activity in vitro. In the present study, chemically synthetized enterocin B (EntB) and the strain E. faecium L3, known as the producer of this peptide, were tested for activity against influenza viruses. The inhibition of cytopathic effect of А/Perth/16/2009(H3N2) and A/South Africa/3626/2013(H1N1) pdm influenza viruses in MDCK cells by chemically synthetized EntB was revealed. The EntB demonstrated antiviral activity at a concentration of 2.5–5 μg/ml depending on the dose of viruses. This peptide exhibited low toxicity in MDCK cells, causing partial damage of the monolayer of the cells only at a concentration above 10 μg/ml. It was also shown, that strain E. faecium L3-protected mice from lethal A/South Africa/3626/2013(H1N1) pdm infection. We speculate that this protective effect of enterococci may be associated with the specific action of enterocin B, which possesses antiviral activity in vitro.",

keywords = "Enterocin B, Enterococcus faecium, Influenza virus",

author = "Ermolenko, {E. I.} and Desheva, {Y. A.} and Kolobov, {A. A.} and Kotyleva, {M. P.} and Sychev, {I. A.} and Suvorov, {A. N.}",

year = "2018",

doi = "10.1007/s12602-018-9457-0",

language = "English",

volume = "11",

pages = "705--712",

journal = "Probiotics and Antimicrobial Proteins",

issn = "1867-1306",

publisher = "Springer Nature",

number = "2",

}

RIS

TY - JOUR

T1 - Anti-Influenza Activity of Enterocin B In vitro and Protective Effect of Bacteriocinogenic Enterococcal Probiotic Strain on Influenza Infection in Mouse Model.

AU - Ermolenko, E. I.

AU - Desheva, Y. A.

AU - Kolobov, A. A.

AU - Kotyleva, M. P.

AU - Sychev, I. A.

AU - Suvorov, A. N.

PY - 2018

Y1 - 2018

N2 - In spite of scientific evidence demonstrating the antiviral activity of lactic-acids bacteria, little is known about the mechanism of their action. Previously, several bacteriocins isolated from lactic acid bacteria (LAB) and some other microorganisms were reported as having antiviral activity in vitro. In the present study, chemically synthetized enterocin B (EntB) and the strain E. faecium L3, known as the producer of this peptide, were tested for activity against influenza viruses. The inhibition of cytopathic effect of А/Perth/16/2009(H3N2) and A/South Africa/3626/2013(H1N1) pdm influenza viruses in MDCK cells by chemically synthetized EntB was revealed. The EntB demonstrated antiviral activity at a concentration of 2.5–5 μg/ml depending on the dose of viruses. This peptide exhibited low toxicity in MDCK cells, causing partial damage of the monolayer of the cells only at a concentration above 10 μg/ml. It was also shown, that strain E. faecium L3-protected mice from lethal A/South Africa/3626/2013(H1N1) pdm infection. We speculate that this protective effect of enterococci may be associated with the specific action of enterocin B, which possesses antiviral activity in vitro.

AB - In spite of scientific evidence demonstrating the antiviral activity of lactic-acids bacteria, little is known about the mechanism of their action. Previously, several bacteriocins isolated from lactic acid bacteria (LAB) and some other microorganisms were reported as having antiviral activity in vitro. In the present study, chemically synthetized enterocin B (EntB) and the strain E. faecium L3, known as the producer of this peptide, were tested for activity against influenza viruses. The inhibition of cytopathic effect of А/Perth/16/2009(H3N2) and A/South Africa/3626/2013(H1N1) pdm influenza viruses in MDCK cells by chemically synthetized EntB was revealed. The EntB demonstrated antiviral activity at a concentration of 2.5–5 μg/ml depending on the dose of viruses. This peptide exhibited low toxicity in MDCK cells, causing partial damage of the monolayer of the cells only at a concentration above 10 μg/ml. It was also shown, that strain E. faecium L3-protected mice from lethal A/South Africa/3626/2013(H1N1) pdm infection. We speculate that this protective effect of enterococci may be associated with the specific action of enterocin B, which possesses antiviral activity in vitro.

KW - Enterocin B

KW - Enterococcus faecium

KW - Influenza virus

UR - http://www.scopus.com/inward/record.url?scp=85053039832&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/antiinfluenza-activity-enterocin-b-vitro-protective-effect-bacteriocinogenic-enterococcal-probiotic

U2 - 10.1007/s12602-018-9457-0

DO - 10.1007/s12602-018-9457-0

M3 - Article

VL - 11

SP - 705

EP - 712

JO - Probiotics and Antimicrobial Proteins

JF - Probiotics and Antimicrobial Proteins

SN - 1867-1306

IS - 2

ER -

ID: 33810365