Research output: Contribution to journal › Article › peer-review
Antibacterial and osteogenic properties of Ag nanoparticles and Ag/TiO2 nanostructures prepared by atomic layer deposition. / Назаров, Денис Васильевич; Ежов, Илья; Юдинцева, Наталья; Шевцов, Максим ; Рудакова, Аида Витальевна; Калганов, Владимир Дмитриевич; Толмачев, Владимир; Жарова, Юлия; Lutakov, Oleksiy; Краева, Людмила Александровна; Рогачева, Елизавета; Максимов, Максим.
In: Journal of Functional Biomaterials, Vol. 13, No. 2, 62, 18.05.2022.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Antibacterial and osteogenic properties of Ag nanoparticles and Ag/TiO2 nanostructures prepared by atomic layer deposition
AU - Назаров, Денис Васильевич
AU - Ежов, Илья
AU - Юдинцева, Наталья
AU - Шевцов, Максим
AU - Рудакова, Аида Витальевна
AU - Калганов, Владимир Дмитриевич
AU - Толмачев, Владимир
AU - Жарова, Юлия
AU - Lutakov, Oleksiy
AU - Краева, Людмила Александровна
AU - Рогачева, Елизавета
AU - Максимов, Максим
N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5/18
Y1 - 2022/5/18
N2 - The combination of titania nanofilms and silver nanoparticles (NPs) is a very promising material, with antibacterial and osseointegration-induced properties for titanium implant coatings. In this work, we successfully prepared TiO2 nanolayer/Ag NP structures on titanium disks using atomiclayer deposition (ALD). The samples were studied by scanning electron microscopy (SEM), X-ray diffraction, X-ray photoelectron spectroscopy (XPS), contact angle measurements, and SEM-EDS.Antibacterial activity was tested against Staphylococcus aureus. The in vitro cytological response of MG-63 osteosarcoma and human fetal mesenchymal stem cells (FetMSCs) was examined using SEMstudy of their morphology, MTT test of viability and differentiation using alkaline phosphataseand osteopontin with and without medium-induced differentiation in the osteogenic direction. The samples with TiO2 nanolayers, Ag NPs, and a TiO2/Ag combination showed high antibacterial activity, differentiation in the osteogenic direction, and non-cytotoxicity. The medium for differentiation significantly improved osteogenic differentiation, but the ALD coatings also stimulated differentiation in the absence of the medium. The TiO2/Ag samples showed the best antibacterial ability and differentiation in the osteogenic direction, indicating the success of the combining of TiO2 and Ag to produce a multifunctional biocompatible and bactericidal material.
AB - The combination of titania nanofilms and silver nanoparticles (NPs) is a very promising material, with antibacterial and osseointegration-induced properties for titanium implant coatings. In this work, we successfully prepared TiO2 nanolayer/Ag NP structures on titanium disks using atomiclayer deposition (ALD). The samples were studied by scanning electron microscopy (SEM), X-ray diffraction, X-ray photoelectron spectroscopy (XPS), contact angle measurements, and SEM-EDS.Antibacterial activity was tested against Staphylococcus aureus. The in vitro cytological response of MG-63 osteosarcoma and human fetal mesenchymal stem cells (FetMSCs) was examined using SEMstudy of their morphology, MTT test of viability and differentiation using alkaline phosphataseand osteopontin with and without medium-induced differentiation in the osteogenic direction. The samples with TiO2 nanolayers, Ag NPs, and a TiO2/Ag combination showed high antibacterial activity, differentiation in the osteogenic direction, and non-cytotoxicity. The medium for differentiation significantly improved osteogenic differentiation, but the ALD coatings also stimulated differentiation in the absence of the medium. The TiO2/Ag samples showed the best antibacterial ability and differentiation in the osteogenic direction, indicating the success of the combining of TiO2 and Ag to produce a multifunctional biocompatible and bactericidal material.
KW - antibacterial properties
KW - atomic layer deposition
KW - cell differentiation
KW - cell viability
KW - mesenchymal stromal cells
KW - nanoparticles
KW - silver
KW - thin films
KW - titania
UR - https://www.mdpi.com/2079-4983/13/2/62/htm
UR - https://www.mendeley.com/catalogue/d324285e-18e1-3316-b89b-7a0645ffde17/
UR - http://www.scopus.com/inward/record.url?scp=85130959443&partnerID=8YFLogxK
U2 - 10.3390/jfb13020062
DO - 10.3390/jfb13020062
M3 - Article
VL - 13
JO - Journal of Functional Biomaterials
JF - Journal of Functional Biomaterials
SN - 2079-4983
IS - 2
M1 - 62
ER -
ID: 95021801