Research output: Contribution to journal › Article › peer-review
Anthocyanin Composition and Content in Rye Plants with Different Grain Color. / Zykin, Pavel A.; Andreeva, Elena A.; Lykholay, Anna N.; Tsvetkova, Natalia V.; Voylokov, Anatoly V.
In: Molecules, Vol. 23, No. 4, 948, 04.2018.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Anthocyanin Composition and Content in Rye Plants with Different Grain Color
AU - Zykin, Pavel A.
AU - Andreeva, Elena A.
AU - Lykholay, Anna N.
AU - Tsvetkova, Natalia V.
AU - Voylokov, Anatoly V.
PY - 2018/4
Y1 - 2018/4
N2 - The color of grain in cereals is determined mainly by anthocyanin pigments. A large level of genetic diversity for anthocyanin content and composition in the grain of different species was observed. In rye, recessive mutations in six genes (vi1...vi6) lead to the absence of anthocyanins in all parts of the plant. Moreover, dominant genes of anthocyanin synthesis in aleurone (gene C) and pericarp (gene Vs) also affect the color of the grain. Reverse phase high-performance liquid chromatography and mass spectrometry were used to study anthocyanins in 24 rye samples. A lack of anthocyanins in the lines with yellow and brown grain was determined. Delphinidin rutinoside and cyanidin rutinoside were found in the green-seeded lines. Six samples with violet grains significantly varied in terms of anthocyanin composition and content. However, the main aglycone was cyanidin or peonidin in all of them. Monosaccharide glucose and disaccharide rutinose served as the glycoside units. Violet-seeded accession forms differ in the ratio of the main anthocyanins and the range of their acylated derivatives. The acyl groups were presented mainly by radicals of malonic and sinapic acids. For the colored forms, a profile of the revealed anthocyanins with the indication of their contents was given. The obtained results are discussed in connection to similar data in rice, barley, and wheat, which will provide a perspective for future investigations.
AB - The color of grain in cereals is determined mainly by anthocyanin pigments. A large level of genetic diversity for anthocyanin content and composition in the grain of different species was observed. In rye, recessive mutations in six genes (vi1...vi6) lead to the absence of anthocyanins in all parts of the plant. Moreover, dominant genes of anthocyanin synthesis in aleurone (gene C) and pericarp (gene Vs) also affect the color of the grain. Reverse phase high-performance liquid chromatography and mass spectrometry were used to study anthocyanins in 24 rye samples. A lack of anthocyanins in the lines with yellow and brown grain was determined. Delphinidin rutinoside and cyanidin rutinoside were found in the green-seeded lines. Six samples with violet grains significantly varied in terms of anthocyanin composition and content. However, the main aglycone was cyanidin or peonidin in all of them. Monosaccharide glucose and disaccharide rutinose served as the glycoside units. Violet-seeded accession forms differ in the ratio of the main anthocyanins and the range of their acylated derivatives. The acyl groups were presented mainly by radicals of malonic and sinapic acids. For the colored forms, a profile of the revealed anthocyanins with the indication of their contents was given. The obtained results are discussed in connection to similar data in rice, barley, and wheat, which will provide a perspective for future investigations.
KW - Anthocyanins/analysis
KW - Chromatography, High Pressure Liquid
KW - Mass Spectrometry
KW - Oryza/chemistry
KW - Pigmentation
KW - Secale/chemistry
KW - Triticum/chemistry
KW - Zea mays/chemistry
KW - L.
KW - HPLC-MS analysis
KW - BLUE
KW - BLACK RICE
KW - comparative genetics
KW - PURPLE
KW - WHEAT
KW - IDENTIFICATION
KW - anthocyanin identification
KW - rye inbred lines
KW - FLAVONOID BIOSYNTHESIS
KW - genes of grain color
KW - PERICARP
KW - BARLEY
KW - GENES
UR - http://www.scopus.com/inward/record.url?scp=85045666865&partnerID=8YFLogxK
U2 - 10.3390/molecules23040948
DO - 10.3390/molecules23040948
M3 - Article
C2 - 29671758
AN - SCOPUS:85045666865
VL - 23
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 4
M1 - 948
ER -
ID: 25418443