Sarcoidosis is a granulomatous disease of unknown etiology. The role of B-cells in pathogene-sis of sarcoidosis was shown in several studies. The importance of B-lymphocytes activation in sarcoidosis can be confirmed by the efficacy of anti-CD20 treatment. However, there is no data about alteration of certain B-cells subsets. In this study changes in subsets of B-lymphocytes in patients with sarcoidosis (n = 37) and a control group (n = 30) are analyzed. In patients with sarcoidosis misbalance of B-naïve and B-memory cells was found, the level of B-naïve cells proved to be more than 70.00 % and B memory cells less than 30.00 % (sensitivity 76 %, speci-ficity 70 %). Among regulatory B cells there was detected an elevation of CD24+++CD38+++ B cells more than 6.5 % (sensitivity 91 %, specificity 88 %) and CD5+CD27-B cells than 12.45 % (sensitivity 76 %, specificity 80 %). These results show, that in sarcoidosis pathogenesis are in-volved B-naïve and B-memory cells, that is characteristic for granulomatous diseases, and also regulatory B cells, that produce anti-inflammatory cytokines.