To test a person’s psycho-emotional state, various methods are used, one of which is a reaction to an emotional video sequence. Such a reaction can make it possible to assess the degree of a person’s involvement in certain emotional events. However, there are few methods to assess psycho-emotional status objectively. One of the sensitive markers is the analysis of a subject’s electroencephalogram (EEG), requiring appropriate videos and EEG analyses. Here, a unique video sequence was chosen that evokes positive emotions in the first part and empathy/sadness in the final part. Therefore, evaluating the transition dynamics from one emotion to another is possible. The analysis showed that such a transition is reliably reflected in the change in the amplitude of rhythms in the beta and gamma ranges of the EEG, as well as their topography. The proposed approach may be unique for assessing a person's psycho-emotional state dynamics.