DOI

  • Andrej Frolov
  • Anja Henning
  • Christoph Böttcher
  • Alain Tissier
  • Dieter Strack

The seed residues left after pressing of rapeseed oil are rich in proteins and could be used for human nutrition and animal feeding. These press cakes contain, however, antinutritives, with fiber being the most abundant one. The analysis of fiber phenolic component (localized to seed coat cell walls) is, therefore, important in breeding and food quality control. However, correct structure and content assignments of cell wall-bound phenolics are challenging due to their low stability during sample preparation. Here, a novel LC-MS/MS-based method for the simultaneous identification and quantitation of 66 cell wall-bound phenolics and their derivatives is described. The method was internally standardized, corrected for degradation effects during sample preparation, and cross-validated with a well-established UV-based procedure. This approach was successfully applied to the analysis of cell wall phenolic patterns in different B. napus cultivars and proved to be suitable for marker compound search as well as assay development.

Original languageEnglish
Pages (from-to)1219-1227
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number6
DOIs
StatePublished - 13 Feb 2013

    Research areas

  • Brassica napus, cell wall phenolics, information-dependent acquisition (IDA), LC-MS/MS, quantitation, tandem mass spectrometry

    Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

ID: 99372710