An ultrastructural study of opportunistic fungal pathogen Rhizomucor pusillus (Mucoromycetes)

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An ultrastructural study of opportunistic fungal pathogen Rhizomucor pusillus (Mucoromycetes). / Avdeenko, Yu. L. ; Vishnyakov, A. E. ; Ivanova, A. N.; Taraskina, A. E. ; Chilina, G. A. ; Bosak , I. A. ; Bogomolova, T. S. ; Karpov, S. A. .

In: МИКОЛОГИЯ И ФИТОПАТОЛОГИЯ, Vol. 55, No. 6, 12.2021, p. 387–395.

Research output: Contribution to journal › Article › peer-review

Author

Avdeenko, Yu. L. ; Vishnyakov, A. E. ; Ivanova, A. N. ; Taraskina, A. E. ; Chilina, G. A. ; Bosak , I. A. ; Bogomolova, T. S. ; Karpov, S. A. . / An ultrastructural study of opportunistic fungal pathogen Rhizomucor pusillus (Mucoromycetes). In: МИКОЛОГИЯ И ФИТОПАТОЛОГИЯ. 2021 ; Vol. 55, No. 6. pp. 387–395.

BibTeX

@article{bf68da709c9f4f2eb11241264ae9746d,

title = "An ultrastructural study of opportunistic fungal pathogen Rhizomucor pusillus (Mucoromycetes)",

abstract = "Mucoromycetes are well-known cosmopolitan molds. Recently, these fungi have become increasingly important due to their ability to cause life-threatening infections in immunocompromised persons. This paper is the first one to study the ultrastructure of hyphae and sporangiospores in Rhizomucor pusillus, using the strain RCPF 1845. Species identification was done by morphological analysis and confirmed by DNA sequencing of the large subunit ribosomal RNA gene. For a more comprehensive analysis, transmission electron microscopy was carried out with specimens, fixed by chemical and cryofixation with high pressure freezing procedure. High pressure cryofixation preserved microtubules in the cytoplasm and nucleoplasm better, although traditional chemical fixation using paraform allowed to get the acceptable images of sporangiospores. We described the structure of nuclei, cell walls and septa, mitochondria and autophagic vacuoles. New data have been obtained on the vesicular transport through the hyphal wall.",

keywords = "cryofixation, mucoromycetes, ultrastructure, vesicular transport",

author = "Avdeenko, {Yu. L.} and Vishnyakov, {A. E.} and Ivanova, {A. N.} and Taraskina, {A. E.} and Chilina, {G. A.} and Bosak, {I. A.} and Bogomolova, {T. S.} and Karpov, {S. A.}",

year = "2021",

month = dec,

doi = "10.31857/s0026364821060040",

language = "English",

volume = "55",

pages = "387–395",

journal = "МИКОЛОГИЯ И ФИТОПАТОЛОГИЯ",

issn = "0026-3648",

publisher = "Российская академия наук",

number = "6",

}

RIS

TY - JOUR

T1 - An ultrastructural study of opportunistic fungal pathogen Rhizomucor pusillus (Mucoromycetes)

AU - Avdeenko, Yu. L.

AU - Vishnyakov, A. E.

AU - Ivanova, A. N.

AU - Taraskina, A. E.

AU - Chilina, G. A.

AU - Bosak , I. A.

AU - Bogomolova, T. S.

AU - Karpov, S. A.

PY - 2021/12

Y1 - 2021/12

N2 - Mucoromycetes are well-known cosmopolitan molds. Recently, these fungi have become increasingly important due to their ability to cause life-threatening infections in immunocompromised persons. This paper is the first one to study the ultrastructure of hyphae and sporangiospores in Rhizomucor pusillus, using the strain RCPF 1845. Species identification was done by morphological analysis and confirmed by DNA sequencing of the large subunit ribosomal RNA gene. For a more comprehensive analysis, transmission electron microscopy was carried out with specimens, fixed by chemical and cryofixation with high pressure freezing procedure. High pressure cryofixation preserved microtubules in the cytoplasm and nucleoplasm better, although traditional chemical fixation using paraform allowed to get the acceptable images of sporangiospores. We described the structure of nuclei, cell walls and septa, mitochondria and autophagic vacuoles. New data have been obtained on the vesicular transport through the hyphal wall.

AB - Mucoromycetes are well-known cosmopolitan molds. Recently, these fungi have become increasingly important due to their ability to cause life-threatening infections in immunocompromised persons. This paper is the first one to study the ultrastructure of hyphae and sporangiospores in Rhizomucor pusillus, using the strain RCPF 1845. Species identification was done by morphological analysis and confirmed by DNA sequencing of the large subunit ribosomal RNA gene. For a more comprehensive analysis, transmission electron microscopy was carried out with specimens, fixed by chemical and cryofixation with high pressure freezing procedure. High pressure cryofixation preserved microtubules in the cytoplasm and nucleoplasm better, although traditional chemical fixation using paraform allowed to get the acceptable images of sporangiospores. We described the structure of nuclei, cell walls and septa, mitochondria and autophagic vacuoles. New data have been obtained on the vesicular transport through the hyphal wall.

KW - cryofixation

KW - mucoromycetes

KW - ultrastructure

KW - vesicular transport

UR - https://sciencejournals.ru/view-article/?j=mikfit&y=2021&v=55&n=6&a=MikFit2106004Avdeenko

UR - https://www.mendeley.com/catalogue/347ea156-fb7e-32b9-81a7-8265d417495a/

U2 - 10.31857/s0026364821060040

DO - 10.31857/s0026364821060040

M3 - Article

AN - SCOPUS:85135291116

VL - 55

SP - 387

EP - 395

JO - МИКОЛОГИЯ И ФИТОПАТОЛОГИЯ

JF - МИКОЛОГИЯ И ФИТОПАТОЛОГИЯ

SN - 0026-3648

IS - 6

ER -

ID: 87633762