Research output: Contribution to journal › Article › peer-review
An electrochemical biosensor for direct detection of hepatitis C virus. / Antipchik, Mariia; Korzhikova-Vlakh, Evgenia; Polyakov, Dmitry; Tarasenko, Irina; Reut, Jekaterina; Öpik, Andres; Syritski, Vitali.
In: Analytical Biochemistry, Vol. 624, 114196, 01.07.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - An electrochemical biosensor for direct detection of hepatitis C virus
AU - Antipchik, Mariia
AU - Korzhikova-Vlakh, Evgenia
AU - Polyakov, Dmitry
AU - Tarasenko, Irina
AU - Reut, Jekaterina
AU - Öpik, Andres
AU - Syritski, Vitali
N1 - Funding Information: This work was supported by the European Regional Development Fund and the programme Mobilitas Pluss (grant MOBJD489 ) and Estonian Research Council grant PRG307 . Publisher Copyright: © 2021 Elsevier Inc. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/7/1
Y1 - 2021/7/1
N2 - This paper is aimed at the development of a biosensor for direct detection of Hepatitis C virus (HCV) surface antigen: envelope protein (E2). A recombinant LEL fragment of biological cell receptor CD81 and two short synthetic peptides imitating the fragment of LEL sequence of CD81 (linear and loop-like peptides) capable of specific binding to E2 were tested as molecular recognition elements of the biosensor. For this purpose the selected ligands were immobilized to the surface of a screen-printed electrode utilized as an electrochemical sensor platform. The immobilization parameters such as the ligand concentration and the immobilization time were carefully optimized for each ligand. Differential pulse voltammetry used to evaluate quantitatively binding of E2 to the ligands revealed their similar binding affinity towards E2. Thus, the linear peptide was selected as a less expensive and easily prepared ligand for the HCV biosensor preparation. The resulting HCV biosensor demonstrated selectivity towards E2 in the presence of interfering protein, conalbumin. Moreover, it was found that the prepared biosensor effectively detected E2 bound to hepatitis C virus-mimetic particles (HC VMPs) at LOD value of 2.1∙10−5 mg/mL both in 0.01 M PBS solution (pH 7.4) and in simulated blood plasma.
AB - This paper is aimed at the development of a biosensor for direct detection of Hepatitis C virus (HCV) surface antigen: envelope protein (E2). A recombinant LEL fragment of biological cell receptor CD81 and two short synthetic peptides imitating the fragment of LEL sequence of CD81 (linear and loop-like peptides) capable of specific binding to E2 were tested as molecular recognition elements of the biosensor. For this purpose the selected ligands were immobilized to the surface of a screen-printed electrode utilized as an electrochemical sensor platform. The immobilization parameters such as the ligand concentration and the immobilization time were carefully optimized for each ligand. Differential pulse voltammetry used to evaluate quantitatively binding of E2 to the ligands revealed their similar binding affinity towards E2. Thus, the linear peptide was selected as a less expensive and easily prepared ligand for the HCV biosensor preparation. The resulting HCV biosensor demonstrated selectivity towards E2 in the presence of interfering protein, conalbumin. Moreover, it was found that the prepared biosensor effectively detected E2 bound to hepatitis C virus-mimetic particles (HC VMPs) at LOD value of 2.1∙10−5 mg/mL both in 0.01 M PBS solution (pH 7.4) and in simulated blood plasma.
KW - CD81 cell receptor
KW - E2 envelope protein
KW - Electrochemical biosensor
KW - Hepatitis C virus (HCV) detection
KW - Screen printed electrode (SPE)C virus-Mimetic particles (HC VMPs)
KW - Synthetic peptides
KW - Biosensing Techniques/methods
KW - Hepatitis C Antigens/analysis
KW - Humans
KW - Viral Envelope Proteins/analysis
KW - Conalbumin/metabolism
KW - Hepacivirus/isolation & purification
KW - Hepatitis C/blood
KW - Protein Binding
KW - Ligands
KW - Electrochemical Techniques/methods
KW - Antigens, CD/analysis
KW - SYSTEM
KW - particles (HC VMPs)
KW - SOLID-PHASE
KW - RECEPTOR
KW - ELEMENTS
KW - DIAGNOSIS
KW - BINDING
KW - Screen printed electrode (SPE)C virus-Mimetic
UR - http://www.scopus.com/inward/record.url?scp=85104120728&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2021.114196
DO - 10.1016/j.ab.2021.114196
M3 - Article
C2 - 33848501
AN - SCOPUS:85104120728
VL - 624
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
M1 - 114196
ER -
ID: 77721675