An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures

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An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures. / Kachkin, Daniel V. ; Khorolskaya, Julia I. ; Ivanova, Julia S. ; Rubel, Aleksandr A. .

In: Methods and Protocols, Vol. 3, No. 4, 69, 12.2020.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{481f94d0a340498e8df9c5ebeecf3527,

title = "An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures",

abstract = "n this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell culture HEK293T but can be adapted for other cell cultures. This protocol was designed to be simple to execute and cheap since it requires only materials and consumables widely available in molecular laboratories, such as salts, alcohols, etc., and no complicated laboratory equipment. These protocols are highly effective and can be performed in any standard molecular biology laboratory.",

keywords = "plasmid, mammalian cell transfection, lentiviral transduction, lentivirus, HEK293T, E. coli, Plasmid, Mammalian cell transfection, Lentiviral transduction, Lentivirus",

author = "Kachkin, {Daniel V.} and Khorolskaya, {Julia I.} and Ivanova, {Julia S.} and Rubel, {Aleksandr A.}",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = dec,

doi = "10.3390/mps3040069",

language = "English",

volume = "3",

journal = "Methods and Protocols — Open Access Journal ",

issn = "2409-9279",

publisher = "MDPI AG",

number = "4",

}

RIS

TY - JOUR

T1 - An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures

AU - Kachkin, Daniel V.

AU - Khorolskaya, Julia I.

AU - Ivanova, Julia S.

AU - Rubel, Aleksandr A.

PY - 2020/12

Y1 - 2020/12

N2 - n this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell culture HEK293T but can be adapted for other cell cultures. This protocol was designed to be simple to execute and cheap since it requires only materials and consumables widely available in molecular laboratories, such as salts, alcohols, etc., and no complicated laboratory equipment. These protocols are highly effective and can be performed in any standard molecular biology laboratory.

AB - n this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell culture HEK293T but can be adapted for other cell cultures. This protocol was designed to be simple to execute and cheap since it requires only materials and consumables widely available in molecular laboratories, such as salts, alcohols, etc., and no complicated laboratory equipment. These protocols are highly effective and can be performed in any standard molecular biology laboratory.

KW - plasmid

KW - mammalian cell transfection

KW - lentiviral transduction

KW - lentivirus

KW - HEK293T

KW - E. coli

KW - Plasmid

KW - Mammalian cell transfection

KW - Lentiviral transduction

KW - Lentivirus

UR - https://www.mdpi.com/2409-9279/3/4/69

UR - http://www.scopus.com/inward/record.url?scp=85094848468&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/942d92c1-80db-339a-87f0-a4962b100666/

U2 - 10.3390/mps3040069

DO - 10.3390/mps3040069

M3 - Article

VL - 3

JO - Methods and Protocols — Open Access Journal

JF - Methods and Protocols — Open Access Journal

SN - 2409-9279

IS - 4

M1 - 69

ER -

ID: 70046977