TY - JOUR

T1 - An automated salting-out assisted liquid-liquid microextraction approach using 1-octylamine: On-line separation of tetracycline in urine samples followed by HPLC-UV determination

AU - Вах, Кристина

AU - Булатов, Андрей Васильевич

AU - Черкашина, Ксения Дмитриевна

AU - Москвин, Леонид Николаевич

AU - Лезов, Алексей Андреевич

AU - Лебединец, Софья Андреевна

AU - Почивалов, Алексей Сергеевич

N1 - Funding Information: This work was financially supported by the Russian Scientific Foundation (project no. 16-13-10117 ). Ksenia Cherkashina gratefully acknowledges financial support for research in the resource centers from Russian Foundation for Basic Research (research projects No. 16–33-50257 ). Scientific research was partially performed at the Center for Chemical Analysis and Materials Research and the Educational Resource Center of the Research Park of St. Petersburg State University. Appendix A

PY - 2018/7/1

Y1 - 2018/7/1

N2 - An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid

AB - An automated salting-out assisted liquid-liquid microextraction (SALLME) procedure based on a flow system was developed as new approach for pretreatment of complex sample matrix. In this procedure 1-octylamine was investigated as novel extractant for the SALLME. The procedure involved aspiration of the 1-octylamine and sample solution into a mixing chamber of a flow system followed by their air-bubble mixing resulting to isotropic solution formation. To provide phase separation a salting-out agent solution was added into the mixing chamber. After phase separation, the micellar 1-octylamine phase containing analyte was mixed with methanol and transported to a HPLC-UV system. To demonstrate the efficiency of the suggested approach, the automated procedure was applied for the HPLC-UV determination of tetracycline as a proof-of-concept analyte in human urine samples. Under the optimal conditions, the detector response of the analytes was linear in the concentration ranges of 0.5–20 mg L−1. The limit of detection, calculated from a blank test based on 3σ, was 0.17 mg L−1. The results demonstrate that the developed approach is highly cost-effective, simple and rapid

KW - 1-Octylamine, Flow analysis, HPLC-UV, Salting-out assisted liquid-liquid microextraction, Tetracycline, urine

KW - 1-Octylamine

KW - Flow analysis

KW - HPLC-UV

KW - Salting-out assisted liquid-liquid microextraction

KW - Tetracycline

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=85043348985&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2018.02.112

DO - 10.1016/j.talanta.2018.02.112

M3 - Article

VL - 184

SP - 122

EP - 127

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - 1 July

ER -