TY - JOUR

T1 - An Approach to Better Estimation of the Initial Velocity of Enzyme Reactions

AU - Malygina, N. M.

AU - Petrova, T. A.

AU - Lianguzov, A. Y.

AU - Ivanov, A. M.

N1 - Malygina, N.M., Petrova, T.A., Lianguzov, A.Y. et al. Dokl Biochem Biophys (2019) 488: 311. https://doi.org/10.1134/S1607672919050053

PY - 2019

Y1 - 2019

N2 - A new algorithm for computation of the initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce the systematic error of measurements to the minimum, to estimate the reaction velocity in test samples regardless of the enzyme activity levels, and to minimize the assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag phase in the kinetic curve.

AB - A new algorithm for computation of the initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce the systematic error of measurements to the minimum, to estimate the reaction velocity in test samples regardless of the enzyme activity levels, and to minimize the assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag phase in the kinetic curve.

KW - enzyme kinetics, initial reaction velocity, salivary alpha-amylase

UR - https://link.springer.com/article/10.1134/S1607672919050053

M3 - Article

VL - 488

SP - 311

EP - 312

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - 1

ER -