Research output: Contribution to journal › Article › peer-review
Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells. / Osipova, Olga ; Sharoyko, Vladimir ; Zashikhina, Natalia ; Zakharova, Natalya ; Tennikova , Tatiana ; Urtti, Arto ; Korzhikova-Vlakh, Evgenia .
In: Pharmaceutics, Vol. 12, No. 1, 39, 01.2020.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells
AU - Osipova, Olga
AU - Sharoyko, Vladimir
AU - Zashikhina, Natalia
AU - Zakharova, Natalya
AU - Tennikova , Tatiana
AU - Urtti, Arto
AU - Korzhikova-Vlakh, Evgenia
N1 - Funding Information: Funding: The financial support was provided by Government of Russian Federation (Megagrant #14.W03.31.0025).
PY - 2020/1
Y1 - 2020/1
N2 - Polyethyleneimine, poly-L-lysine, chitosan and some others cationic polymers have been thoroughly studied as nucleic acid delivery systems in gene therapy. However, the drug release from these systems proceeds at a very low rate due to extremely high binding between a carrier and gene material. To reduce these interactions and to enhance drug release, we developed a set of amphiphilic polypeptides containing positively and negatively charged amino acids as well as a hydrophobic one. The copolymers obtained were characterized by size-exclusion chromatography, static light scattering, HPLC amino acid analysis and 1HNMR spectroscopy. All copolymers formed particles due to a self-assembly in aqueous media. Depending on polypeptide composition, the formation of particles with hydrodynamic diameters from 180 to 900 nm was observed. Stability of polymer particles, loading and release efficiency were carefully studied. Cellular uptake of the particles was efficient and their cytotoxicity was negligible. The application of polymer carriers, containing siRNA, to vascular endothelial growth factor (VEGF-A165) silencing of ARPE-19 cells was successful. The gene silencing was confirmed by suppression of both messenger RNA and protein expression.
AB - Polyethyleneimine, poly-L-lysine, chitosan and some others cationic polymers have been thoroughly studied as nucleic acid delivery systems in gene therapy. However, the drug release from these systems proceeds at a very low rate due to extremely high binding between a carrier and gene material. To reduce these interactions and to enhance drug release, we developed a set of amphiphilic polypeptides containing positively and negatively charged amino acids as well as a hydrophobic one. The copolymers obtained were characterized by size-exclusion chromatography, static light scattering, HPLC amino acid analysis and 1HNMR spectroscopy. All copolymers formed particles due to a self-assembly in aqueous media. Depending on polypeptide composition, the formation of particles with hydrodynamic diameters from 180 to 900 nm was observed. Stability of polymer particles, loading and release efficiency were carefully studied. Cellular uptake of the particles was efficient and their cytotoxicity was negligible. The application of polymer carriers, containing siRNA, to vascular endothelial growth factor (VEGF-A165) silencing of ARPE-19 cells was successful. The gene silencing was confirmed by suppression of both messenger RNA and protein expression.
KW - Amphiphilic polypeptides
KW - Gene silencing
KW - Nanoparticles
KW - Self-assembly
KW - VEGF
KW - siRNA delivery
UR - http://www.scopus.com/inward/record.url?scp=85078562232&partnerID=8YFLogxK
U2 - 10.3390/pharmaceutics12010039
DO - 10.3390/pharmaceutics12010039
M3 - Article
VL - 12
JO - Pharmaceutics
JF - Pharmaceutics
SN - 1999-4923
IS - 1
M1 - 39
ER -
ID: 61444572