Research output: Contribution to journal › Article › peer-review
Activation of Cardiac Stem Cells in Myocardial Infarction. / Docshin, P. M. ; Karpov, A.A.; Eyvazova, Sh. D.; Puzanov, M. V.; Kostareva, A. A.; Galagudza, M. M.; Malashicheva, A. B.
In: Cell and Tissue Biology, Vol. 12, No. 3, 2018, p. 175-182.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Activation of Cardiac Stem Cells in Myocardial Infarction
AU - Docshin, P. M.
AU - Karpov, A.A.
AU - Eyvazova, Sh. D.
AU - Puzanov, M. V.
AU - Kostareva, A. A.
AU - Galagudza, M. M.
AU - Malashicheva, A. B.
N1 - Docshin, P.M., Karpov, A.A., Eyvazova, S.D. et al. Activation of Cardiac Stem Cells in Myocardial Infarction. Cell Tiss. Biol. 12, 175–182 (2018). https://doi.org/10.1134/S1990519X18030045
PY - 2018
Y1 - 2018
N2 - The development of heart failure caused by acute myocardial infarction is accompanied by massive necrotic death of cardiomyocytes in lesion areas and subsequent pathological myocardial remodeling. Traditionally, the possibility of heart reparation has been considered to be severely limited or absent in the postnatal period. Endogenous cardiac stem cells with a regenerative potential have recently been described, but the mechanisms of activation of these cells remain poorly understood. The aim of our work was to obtain cardiac stem cells from the ischemic area of the myocardium and compare their functional properties with stem cells isolated from the healthy area of the myocardium. RT-PCR was used to quantify the gene expression in cardiac stem cells. In addition, differentiated cells were stained for specific markers using immunocytochemical method. Cardiac stem cells originating from the infarction area had a higher proliferative potential and a greater propensity to migrate in comparison to the cells originated from a healthy myocardial area. The expression level of several specific markers of cardiogenic, osteogenic and adipogenic differentiation upon induction of corresponding differentiation was higher in the cells from the infarction area than in cells from the healthy myocardium. We conclude that myocardial ischemia activates the internal regenerative potential of cardiac stem cells.
AB - The development of heart failure caused by acute myocardial infarction is accompanied by massive necrotic death of cardiomyocytes in lesion areas and subsequent pathological myocardial remodeling. Traditionally, the possibility of heart reparation has been considered to be severely limited or absent in the postnatal period. Endogenous cardiac stem cells with a regenerative potential have recently been described, but the mechanisms of activation of these cells remain poorly understood. The aim of our work was to obtain cardiac stem cells from the ischemic area of the myocardium and compare their functional properties with stem cells isolated from the healthy area of the myocardium. RT-PCR was used to quantify the gene expression in cardiac stem cells. In addition, differentiated cells were stained for specific markers using immunocytochemical method. Cardiac stem cells originating from the infarction area had a higher proliferative potential and a greater propensity to migrate in comparison to the cells originated from a healthy myocardial area. The expression level of several specific markers of cardiogenic, osteogenic and adipogenic differentiation upon induction of corresponding differentiation was higher in the cells from the infarction area than in cells from the healthy myocardium. We conclude that myocardial ischemia activates the internal regenerative potential of cardiac stem cells.
KW - cardiac stem cells
KW - mechanisms of activation of heart repair
KW - myocardial infarction
UR - http://www.scopus.com/inward/record.url?scp=85048497939&partnerID=8YFLogxK
U2 - 10.1134/S1990519X18030045
DO - 10.1134/S1990519X18030045
M3 - Article
AN - SCOPUS:85048497939
VL - 12
SP - 175
EP - 182
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 3
ER -
ID: 35806114