Research output: Contribution to journal › Article › peer-review
Annotating newly sequenced genomes and determining alternative isoforms from long-read RNA data are complex and incompletely solved problems. Here we present IsoQuant-a computational tool using intron graphs that accurately reconstructs transcripts both with and without reference genome annotation. For novel transcript discovery, IsoQuant reduces the false-positive rate fivefold and 2.5-fold for Oxford Nanopore reference-based or reference-free mode, respectively. IsoQuant also improves performance for Pacific Biosciences data.
Original language | English |
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Pages (from-to) | 915-918 |
Number of pages | 4 |
Journal | Nature Biotechnology |
Volume | 41 |
Issue number | 7 |
Early online date | 2 Jan 2023 |
DOIs | |
State | Published - Jul 2023 |
ID: 106999301