DOI

  • Alexei Sorokin
  • Alla Lapidus
  • Veronique Capuano
  • Nathalie Galleron
  • Petar Pujic
  • S. Dusko Ehrlich

An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the Bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes.

Original languageEnglish
Pages (from-to)448-453
Number of pages6
JournalGenome Research
Volume6
Issue number5
DOIs
StatePublished - 1996

    Scopus subject areas

  • Genetics
  • Genetics(clinical)

ID: 90043117