Research output: Contribution to journal › Article › peer-review
An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the Bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes.
Original language | English |
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Pages (from-to) | 448-453 |
Number of pages | 6 |
Journal | Genome Research |
Volume | 6 |
Issue number | 5 |
DOIs | |
State | Published - 1996 |
ID: 90043117