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A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’. / Fizikova, A.; Tukhuzheva, Z.; Zhokhova, L.; Tvorogova, V.; Lutova, L.

In: International Journal of Molecular Sciences, Vol. 25, No. 18, 17.09.2024.

Research output: Contribution to journal › Article › peer-review

Harvard

Fizikova, A, Tukhuzheva, Z, Zhokhova, L, Tvorogova, V & Lutova, L 2024, 'A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’', International Journal of Molecular Sciences, vol. 25, no. 18. https://doi.org/10.3390/ijms251810011

APA

Fizikova, A., Tukhuzheva, Z., Zhokhova, L., Tvorogova, V., & Lutova, L. (2024). A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’. International Journal of Molecular Sciences, 25(18). https://doi.org/10.3390/ijms251810011

Vancouver

Fizikova A, Tukhuzheva Z, Zhokhova L, Tvorogova V, Lutova L. A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’. International Journal of Molecular Sciences. 2024 Sep 17;25(18). https://doi.org/10.3390/ijms251810011

Author

Fizikova, A. ; Tukhuzheva, Z. ; Zhokhova, L. ; Tvorogova, V. ; Lutova, L. / A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’. In: International Journal of Molecular Sciences. 2024 ; Vol. 25, No. 18.

BibTeX

@article{f9fd90f2246545e191549637b88bbee1,
title = "A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. {\textquoteleft}Merlot{\textquoteright}",
abstract = "Grape is one of the most economically significant berry crops. Owing to the biological characteristics of grapes, such as the long juvenile period (5–8 years), high degree of genome heterozygosity, and the frequent occurrence of inbreeding depression, homozygosity during crossbreeding leads to loss of varietal characteristics and viability. CRISPR/Cas editing has become the tool of choice for improving elite technical grape varieties. This study provides the first evidence of a decrease in the total fraction of phenolic compounds and an increase in the concentration of peroxide compounds in grape callus cells upon the addition of chitosan to the culture medium. These previously unreported metabolic features of the grape response to chitosan have been described and used for the first time to increase the probability of selecting plant cells with MLO7 knockout characterised by an oxidative burst in response to the presence of a pathogen modulated by chitosan in the high-metabolite black grape variety {\textquoteleft}Merlot{\textquoteright}. This was achieved by using a CRISPR/Cas9 editing vector construction with the peroxide sensor HyPer as a reporter. This research represents the first CRISPR/Cas9 editing of {\textquoteleft}Merlot{\textquoteright}, one of the most economically important elite technical grape varieties. {\textcopyright} 2024 by the authors.",
keywords = "CRISPR/Cas9, grape editing, mildew, plant biotechnology, plant resistance, chitosan, hypochlorite sodium, peroxide, phenol derivative, plant protein, Article, bacterial strain, berry, clustered regularly interspaced short palindromic repeat, controlled study, CRISPR-CAS9 system, cross breeding, gene editing, genotyping, grape, heterozygosity, homozygosity, inbreeding depression, infectious agent, metabolite, micropropagation, nonhuman, phenotype, plant cell, plant immunity, plant leaf, plasmid, protoplast, reporter gene, respiratory burst, CRISPR Cas system, disease resistance, genetics, metabolism, microbiology, plant disease, procedures, Vitis, wine, Chitosan, CRISPR-Cas Systems, Disease Resistance, Gene Editing, Phenols, Plant Cells, Plant Diseases, Plant Proteins, Wine, Gene Editing/methods, Chitosan/pharmacology, Plant Diseases/genetics, Plant Proteins/genetics, Disease Resistance/genetics, Vitis/genetics, Plant Cells/metabolism",
author = "A. Fizikova and Z. Tukhuzheva and L. Zhokhova and V. Tvorogova and L. Lutova",
note = "Export Date: 19 October 2024 Адрес для корреспонденции: Fizikova, A.; Plant Biology and Biotechnology Department, Olympic Avenue 1, Russian Federation; эл. почта: fizikova.ay@talantiuspeh.ru Химические вещества/CAS: chitosan, 9012-76-4; hypochlorite sodium, 7681-52-9; peroxide, 14915-07-2; Chitosan; Phenols; Plant Proteins",
year = "2024",
month = sep,
day = "17",
doi = "10.3390/ijms251810011",
language = "Английский",
volume = "25",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI AG",
number = "18",

}

RIS

TY - JOUR

T1 - A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’

AU - Fizikova, A.

AU - Tukhuzheva, Z.

AU - Zhokhova, L.

AU - Tvorogova, V.

AU - Lutova, L.

N1 - Export Date: 19 October 2024 Адрес для корреспонденции: Fizikova, A.; Plant Biology and Biotechnology Department, Olympic Avenue 1, Russian Federation; эл. почта: fizikova.ay@talantiuspeh.ru Химические вещества/CAS: chitosan, 9012-76-4; hypochlorite sodium, 7681-52-9; peroxide, 14915-07-2; Chitosan; Phenols; Plant Proteins

PY - 2024/9/17

Y1 - 2024/9/17

N2 - Grape is one of the most economically significant berry crops. Owing to the biological characteristics of grapes, such as the long juvenile period (5–8 years), high degree of genome heterozygosity, and the frequent occurrence of inbreeding depression, homozygosity during crossbreeding leads to loss of varietal characteristics and viability. CRISPR/Cas editing has become the tool of choice for improving elite technical grape varieties. This study provides the first evidence of a decrease in the total fraction of phenolic compounds and an increase in the concentration of peroxide compounds in grape callus cells upon the addition of chitosan to the culture medium. These previously unreported metabolic features of the grape response to chitosan have been described and used for the first time to increase the probability of selecting plant cells with MLO7 knockout characterised by an oxidative burst in response to the presence of a pathogen modulated by chitosan in the high-metabolite black grape variety ‘Merlot’. This was achieved by using a CRISPR/Cas9 editing vector construction with the peroxide sensor HyPer as a reporter. This research represents the first CRISPR/Cas9 editing of ‘Merlot’, one of the most economically important elite technical grape varieties. © 2024 by the authors.

AB - Grape is one of the most economically significant berry crops. Owing to the biological characteristics of grapes, such as the long juvenile period (5–8 years), high degree of genome heterozygosity, and the frequent occurrence of inbreeding depression, homozygosity during crossbreeding leads to loss of varietal characteristics and viability. CRISPR/Cas editing has become the tool of choice for improving elite technical grape varieties. This study provides the first evidence of a decrease in the total fraction of phenolic compounds and an increase in the concentration of peroxide compounds in grape callus cells upon the addition of chitosan to the culture medium. These previously unreported metabolic features of the grape response to chitosan have been described and used for the first time to increase the probability of selecting plant cells with MLO7 knockout characterised by an oxidative burst in response to the presence of a pathogen modulated by chitosan in the high-metabolite black grape variety ‘Merlot’. This was achieved by using a CRISPR/Cas9 editing vector construction with the peroxide sensor HyPer as a reporter. This research represents the first CRISPR/Cas9 editing of ‘Merlot’, one of the most economically important elite technical grape varieties. © 2024 by the authors.

KW - CRISPR/Cas9

KW - grape editing

KW - mildew

KW - plant biotechnology

KW - plant resistance

KW - chitosan

KW - hypochlorite sodium

KW - peroxide

KW - phenol derivative

KW - plant protein

KW - Article

KW - bacterial strain

KW - berry

KW - clustered regularly interspaced short palindromic repeat

KW - controlled study

KW - CRISPR-CAS9 system

KW - cross breeding

KW - gene editing

KW - genotyping

KW - grape

KW - heterozygosity

KW - homozygosity

KW - inbreeding depression

KW - infectious agent

KW - metabolite

KW - micropropagation

KW - nonhuman

KW - phenotype

KW - plant cell

KW - plant immunity

KW - plant leaf

KW - plasmid

KW - protoplast

KW - reporter gene

KW - respiratory burst

KW - CRISPR Cas system

KW - disease resistance

KW - genetics

KW - metabolism

KW - microbiology

KW - plant disease

KW - procedures

KW - Vitis

KW - wine

KW - Chitosan

KW - CRISPR-Cas Systems

KW - Disease Resistance

KW - Gene Editing

KW - Phenols

KW - Plant Cells

KW - Plant Diseases

KW - Plant Proteins

KW - Wine

KW - Gene Editing/methods

KW - Chitosan/pharmacology

KW - Plant Diseases/genetics

KW - Plant Proteins/genetics

KW - Disease Resistance/genetics

KW - Vitis/genetics

KW - Plant Cells/metabolism

UR - https://www.mendeley.com/catalogue/6259a73e-30a2-3e17-b0de-3e7068bb1cec/

U2 - 10.3390/ijms251810011

DO - 10.3390/ijms251810011

M3 - статья

C2 - 39337500

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 18

ER -

ID: 126386937