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A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids. / Larionov, V. L.; Karpova, T. S.; Kouprina, N. Y.; Jouravleva, G. A.

In: Current Genetics, Vol. 10, No. 1, 10.1985, p. 15-20.

Research output: Contribution to journal › Article › peer-review

Harvard

Larionov, VL, Karpova, TS, Kouprina, NY & Jouravleva, GA 1985, 'A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids', Current Genetics, vol. 10, no. 1, pp. 15-20. https://doi.org/10.1007/BF00418488

APA

Larionov, V. L., Karpova, T. S., Kouprina, N. Y., & Jouravleva, G. A. (1985). A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids. Current Genetics, 10(1), 15-20. https://doi.org/10.1007/BF00418488

Vancouver

Larionov VL, Karpova TS, Kouprina NY, Jouravleva GA. A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids. Current Genetics. 1985 Oct;10(1):15-20. https://doi.org/10.1007/BF00418488

Author

Larionov, V. L. ; Karpova, T. S. ; Kouprina, N. Y. ; Jouravleva, G. A. / A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids. In: Current Genetics. 1985 ; Vol. 10, No. 1. pp. 15-20.

BibTeX

@article{4f8a5b6bd55f4e92806cd542c5ee9b11,
title = "A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids",
abstract = "A mutant with unstable maintenance of hybrid plasmids containing either one of the centromeric loci CEN3, CEN6, CEN11 and arsl or the replicator of the 2 μ plasmid has been obtained. The frequency of loss of hybrid plasmids in the mutant was up to 3 · 10-1 per one generation versus 10-2 in the original strain. The unstable maintenance of minichromosomes in the mutant is controlled by a recessive nuclear gene, named SMC for stability of minichromosomes. Loss of some minichromosomes is connected with impairment of their segregation in cell division. In diploids homozygous for smc mitotic chromosomal segregation is not affected but sporulation is impaired. The question of adequacy of usage of minichromosomes for selection of mutants with impaired function of centromeric loci is discussed.",
keywords = "Centromere-containing plasmids, Mitotic stability of minichromosomes, Saccharomyces cerevisiae, Yeast transformation",
author = "Larionov, {V. L.} and Karpova, {T. S.} and Kouprina, {N. Y.} and Jouravleva, {G. A.}",
year = "1985",
month = oct,
doi = "10.1007/BF00418488",
language = "English",
volume = "10",
pages = "15--20",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Nature",
number = "1",

}

RIS

TY - JOUR

T1 - A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids

AU - Larionov, V. L.

AU - Karpova, T. S.

AU - Kouprina, N. Y.

AU - Jouravleva, G. A.

PY - 1985/10

Y1 - 1985/10

N2 - A mutant with unstable maintenance of hybrid plasmids containing either one of the centromeric loci CEN3, CEN6, CEN11 and arsl or the replicator of the 2 μ plasmid has been obtained. The frequency of loss of hybrid plasmids in the mutant was up to 3 · 10-1 per one generation versus 10-2 in the original strain. The unstable maintenance of minichromosomes in the mutant is controlled by a recessive nuclear gene, named SMC for stability of minichromosomes. Loss of some minichromosomes is connected with impairment of their segregation in cell division. In diploids homozygous for smc mitotic chromosomal segregation is not affected but sporulation is impaired. The question of adequacy of usage of minichromosomes for selection of mutants with impaired function of centromeric loci is discussed.

AB - A mutant with unstable maintenance of hybrid plasmids containing either one of the centromeric loci CEN3, CEN6, CEN11 and arsl or the replicator of the 2 μ plasmid has been obtained. The frequency of loss of hybrid plasmids in the mutant was up to 3 · 10-1 per one generation versus 10-2 in the original strain. The unstable maintenance of minichromosomes in the mutant is controlled by a recessive nuclear gene, named SMC for stability of minichromosomes. Loss of some minichromosomes is connected with impairment of their segregation in cell division. In diploids homozygous for smc mitotic chromosomal segregation is not affected but sporulation is impaired. The question of adequacy of usage of minichromosomes for selection of mutants with impaired function of centromeric loci is discussed.

KW - Centromere-containing plasmids

KW - Mitotic stability of minichromosomes

KW - Saccharomyces cerevisiae

KW - Yeast transformation

UR - http://www.scopus.com/inward/record.url?scp=0022339234&partnerID=8YFLogxK

U2 - 10.1007/BF00418488

DO - 10.1007/BF00418488

M3 - Article

C2 - 3940061

AN - SCOPUS:0022339234

VL - 10

SP - 15

EP - 20

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 1

ER -

ID: 84959294