Research output: Contribution to journal › Article › peer-review
A multi-channel handheld automatic spectrometer for wide range and on-site detection of okadaic acid based on specific aptamer binding. / Ma, Chiyu; Sun, Xianyou; Kong, Liubing; Wang, Xinyi; Zhou, Shuqi; Wei, Xinwei; Kirsanov, Dmitry; Legin, Andrey; Wan, Hao; Wang, Ping.
In: Analytical Methods, Vol. 13, No. 38, 08.10.2021, p. 4345-4353.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A multi-channel handheld automatic spectrometer for wide range and on-site detection of okadaic acid based on specific aptamer binding
AU - Ma, Chiyu
AU - Sun, Xianyou
AU - Kong, Liubing
AU - Wang, Xinyi
AU - Zhou, Shuqi
AU - Wei, Xinwei
AU - Kirsanov, Dmitry
AU - Legin, Andrey
AU - Wan, Hao
AU - Wang, Ping
N1 - Publisher Copyright: © The Royal Society of Chemistry.
PY - 2021/10/8
Y1 - 2021/10/8
N2 - Okadaic acid (OA) is one of the marine toxins that are widely distributed and harmful to humans. However, the current detection methods for OA involve complex procedures, need long detection time, and rely on large-scale laboratory equipment. In this work, a multi-channel handheld automatic spectrometer (MHAS) based on a spectral sensor was developed with the advantages of small size, simple operation and low cost. It could achieve rapid detection within 30 s and a wide spectral detection range of 470-780 nm with a broadband LED as the light source and a microplate containing 8 wells as a sample cell. Moreover, through the combination of gold nanoparticles (AuNPs) and aptamer-OA34, a highly sensitive and rapid system for OA detection was established with a LOD of 1.80 ?g L-1 and a wide detection range of 20-10?000 ?g L-1, which is comparable to a microplate reader. Compared with other studies, the proposed MHAS realized rapid on-site detection of OA with a wider detection range, shorter detection time and higher portability. Therefore, the MHAS promises to be a stable and efficient optical detection instrument for on-site detection in the fields of food safety, disease diagnosis and environmental monitoring. This journal is
AB - Okadaic acid (OA) is one of the marine toxins that are widely distributed and harmful to humans. However, the current detection methods for OA involve complex procedures, need long detection time, and rely on large-scale laboratory equipment. In this work, a multi-channel handheld automatic spectrometer (MHAS) based on a spectral sensor was developed with the advantages of small size, simple operation and low cost. It could achieve rapid detection within 30 s and a wide spectral detection range of 470-780 nm with a broadband LED as the light source and a microplate containing 8 wells as a sample cell. Moreover, through the combination of gold nanoparticles (AuNPs) and aptamer-OA34, a highly sensitive and rapid system for OA detection was established with a LOD of 1.80 ?g L-1 and a wide detection range of 20-10?000 ?g L-1, which is comparable to a microplate reader. Compared with other studies, the proposed MHAS realized rapid on-site detection of OA with a wider detection range, shorter detection time and higher portability. Therefore, the MHAS promises to be a stable and efficient optical detection instrument for on-site detection in the fields of food safety, disease diagnosis and environmental monitoring. This journal is
KW - Gold
KW - Humans
KW - Marine Toxins
KW - Metal Nanoparticles
KW - Okadaic Acid
KW - Refractometry
KW - GOLD NANOPARTICLES
KW - MUSSELS
KW - COLORIMETRIC DETECTION
KW - PLATFORM
KW - DNA
KW - BIOSENSOR
KW - METAL-IONS
KW - SELECTION
UR - http://www.scopus.com/inward/record.url?scp=85117527983&partnerID=8YFLogxK
U2 - 10.1039/d1ay00976a
DO - 10.1039/d1ay00976a
M3 - Article
C2 - 34622887
AN - SCOPUS:85117527983
VL - 13
SP - 4345
EP - 4353
JO - Analytical Methods
JF - Analytical Methods
SN - 1759-9660
IS - 38
ER -
ID: 88217389