TY - JOUR

T1 - НЕЙРОЛЕПТИКИ ПОДАВЛЯЮТ Са2+-ОТВЕТЫ, ВЫЗЫВАЕМЫЕ ГЛУТОКСИМОМ И МОЛИКСАНОМ В МАКРОФАГАХ

AU - Миленина, Лидия Сергеевна

AU - Крутецкая, Зоя Иринарховна

AU - Антонов, Виктор

AU - Крутецкая, Нина Иринарховна

AU - Бадюлина, Валентина Ивановна

AU - Симонян, Армен Оганесович

N1 - Миленина Л.С., Крутецкая З.И., Антонов В.Г., Крутецкая Н.И., Бадюлина В.И., Симонян А.О. Нейролептики подавляют Са2+-ответы, вызываемые глутоксимом и моликсаном в макрофагах. Научный журнал «Актуальные вопросы биологической физики и химии». Севастополь. 2022. Т. 7. № 1. С. 127-136.

PY - 2022/11/8

Y1 - 2022/11/8

N2 - Haloperidol (butyrophenone derivative) and phenothiazine derivatives chlorpromazine and trifluoperazine belong to the typical first-generation neuroleptics and are widely used in clinical practice for treatment of schizophrenia and other mental diseases. Their multifaceted effect on cellular processes is well known. Thus, a high affinity of typical neuroleptics for sigma-1 receptors was revealed. Sigma-1 receptors are ubiquitous multifunctional ligand-operated molecular chaperones in the endoplasmic reticulum membrane with a unique history, structure, and pharmacological profile. Acting as chaperones, sigma-1 receptors modulate a wide range of cellular processes in health and disease, including Ca2+ signaling processes. To elucidate the involvement of sigma-1 receptors in Ca2+ signaling processes in macrophages, the effect of sigma-1 receptor ligands on Ca2+ responses induced by disulfide-containing immunomodulators glutoxim and molixan in rat peritoneal macrophages was investigated. Using Fura-2AM microfluorimetry we have shown that haloperidol, chlorpromazine and trifluoperazine significantly suppress both Ca2+ mobilization from intracellular Ca2+ stores and subsequent store-dependent Ca2+ entry, induced by glutoxim or molixan in peritoneal macrophages. The data obtained indicate the possible involvement of sigma-1 receptors in the complex signaling cascade triggered by glutoxim or molixan and leading to intracellular Ca2+ concentration increase in macrophages. The results also suggest the involvement of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.

AB - Haloperidol (butyrophenone derivative) and phenothiazine derivatives chlorpromazine and trifluoperazine belong to the typical first-generation neuroleptics and are widely used in clinical practice for treatment of schizophrenia and other mental diseases. Their multifaceted effect on cellular processes is well known. Thus, a high affinity of typical neuroleptics for sigma-1 receptors was revealed. Sigma-1 receptors are ubiquitous multifunctional ligand-operated molecular chaperones in the endoplasmic reticulum membrane with a unique history, structure, and pharmacological profile. Acting as chaperones, sigma-1 receptors modulate a wide range of cellular processes in health and disease, including Ca2+ signaling processes. To elucidate the involvement of sigma-1 receptors in Ca2+ signaling processes in macrophages, the effect of sigma-1 receptor ligands on Ca2+ responses induced by disulfide-containing immunomodulators glutoxim and molixan in rat peritoneal macrophages was investigated. Using Fura-2AM microfluorimetry we have shown that haloperidol, chlorpromazine and trifluoperazine significantly suppress both Ca2+ mobilization from intracellular Ca2+ stores and subsequent store-dependent Ca2+ entry, induced by glutoxim or molixan in peritoneal macrophages. The data obtained indicate the possible involvement of sigma-1 receptors in the complex signaling cascade triggered by glutoxim or molixan and leading to intracellular Ca2+ concentration increase in macrophages. The results also suggest the involvement of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.

UR - https://www.mendeley.com/catalogue/5b864ff4-e8ff-3541-a4ad-f50f797f24ed/

U2 - 10.29039/rusjbpc.2022.0494

DO - 10.29039/rusjbpc.2022.0494

M3 - статья

VL - 7

SP - 127

EP - 136

JO - АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ

JF - АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ

SN - 2499-9962

IS - 1

T2 - XVII Международная научная<br/>конференция «Актуальные вопросы биологической физики и химии. БФФХ-2022»

Y2 - 19 September 2022 through 23 September 2022

ER -