Research output: Contribution to journal › Article › peer-review
СРАВНЕНИЕ МЕТОДОВ ТАКСОНОМИЧЕСКОГО ОПРЕДЕЛЕНИЯ БАКТЕРИЙ ПО ПОСЛЕДОВАТЕЛЬНОСТИ ГЕНА 16S С ИСПОЛЬЗОВАНИЕМ ТЕХНОЛОГИЙ СЕКВЕНИРОВАНИЯ ILLUMINA И NANOPORE НА ПРИМЕРЕ НЕФТЯНЫХ ОБРАЗЦОВ. / Бурлаченко, Анастасия Сергеевна ; Некрасова, Дарья Алексеевна; Донников, Михаил Юрьевич; Ермаков, Артем Михайлович; Ждянова, Елизавета Сергеевна; Коваленко, Людмила Васильевна; Данилов, Лаврентий Глебович; Глотов, Олег Сергеевич.
In: ВЕСТНИК СУРГУ. МЕДИЦИНА, Vol. 18, No. 2, 2025, p. 81-87.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - СРАВНЕНИЕ МЕТОДОВ ТАКСОНОМИЧЕСКОГО ОПРЕДЕЛЕНИЯ БАКТЕРИЙ ПО ПОСЛЕДОВАТЕЛЬНОСТИ ГЕНА 16S С ИСПОЛЬЗОВАНИЕМ ТЕХНОЛОГИЙ СЕКВЕНИРОВАНИЯ ILLUMINA И NANOPORE НА ПРИМЕРЕ НЕФТЯНЫХ ОБРАЗЦОВ
AU - Бурлаченко, Анастасия Сергеевна
AU - Некрасова, Дарья Алексеевна
AU - Донников, Михаил Юрьевич
AU - Ермаков, Артем Михайлович
AU - Ждянова, Елизавета Сергеевна
AU - Коваленко, Людмила Васильевна
AU - Данилов, Лаврентий Глебович
AU - Глотов, Олег Сергеевич
PY - 2025
Y1 - 2025
N2 - The use of modern sequencing methods enables us to determine the taxonomic biodiversity of various bacterial communities. Specifically, it allows to assess the diversity of bacterial strains that are not cultured. At present, there are two generations of sequencing technologies that enable the determination of microbial community diversity: 2nd generation (represented by Illumina and BGI) and 3rd generation (Nanopore and PacBio). However, as these technologies use different approaches to sequencing, there is a question of reproducibility of the results. This research revealed that using Illumina and Nanopore sequencing methods differs in terms of diversity indices and taxonomic diversity. This data emphasizes the requirement to develop advanced methods for sample preparation and DNA sequencing to achieve an unequivocal microbial community profile.
AB - The use of modern sequencing methods enables us to determine the taxonomic biodiversity of various bacterial communities. Specifically, it allows to assess the diversity of bacterial strains that are not cultured. At present, there are two generations of sequencing technologies that enable the determination of microbial community diversity: 2nd generation (represented by Illumina and BGI) and 3rd generation (Nanopore and PacBio). However, as these technologies use different approaches to sequencing, there is a question of reproducibility of the results. This research revealed that using Illumina and Nanopore sequencing methods differs in terms of diversity indices and taxonomic diversity. This data emphasizes the requirement to develop advanced methods for sample preparation and DNA sequencing to achieve an unequivocal microbial community profile.
UR - https://www.mendeley.com/catalogue/5c6f2c74-2300-3778-9fa7-ac39ed79e521/
U2 - 10.35266/2949-3447-2025-2-11
DO - 10.35266/2949-3447-2025-2-11
M3 - статья
VL - 18
SP - 81
EP - 87
JO - ВЕСТНИК СУРГУ. МЕДИЦИНА
JF - ВЕСТНИК СУРГУ. МЕДИЦИНА
SN - 2304-9448
IS - 2
ER -
ID: 137823903