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СРАВНЕНИЕ МЕТОДОВ ТАКСОНОМИЧЕСКОГО ОПРЕДЕЛЕНИЯ БАКТЕРИЙ ПО ПОСЛЕДОВАТЕЛЬНОСТИ ГЕНА 16S С ИСПОЛЬЗОВАНИЕМ ТЕХНОЛОГИЙ СЕКВЕНИРОВАНИЯ ILLUMINA И NANOPORE НА ПРИМЕРЕ НЕФТЯНЫХ ОБРАЗЦОВ. / Бурлаченко, Анастасия Сергеевна ; Некрасова, Дарья Алексеевна; Донников, Михаил Юрьевич; Ермаков, Артем Михайлович; Ждянова, Елизавета Сергеевна; Коваленко, Людмила Васильевна; Данилов, Лаврентий Глебович; Глотов, Олег Сергеевич.

In: ВЕСТНИК СУРГУ. МЕДИЦИНА, Vol. 18, No. 2, 2025, p. 81-87.

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Бурлаченко, Анастасия Сергеевна ; Некрасова, Дарья Алексеевна ; Донников, Михаил Юрьевич ; Ермаков, Артем Михайлович ; Ждянова, Елизавета Сергеевна ; Коваленко, Людмила Васильевна ; Данилов, Лаврентий Глебович ; Глотов, Олег Сергеевич. / СРАВНЕНИЕ МЕТОДОВ ТАКСОНОМИЧЕСКОГО ОПРЕДЕЛЕНИЯ БАКТЕРИЙ ПО ПОСЛЕДОВАТЕЛЬНОСТИ ГЕНА 16S С ИСПОЛЬЗОВАНИЕМ ТЕХНОЛОГИЙ СЕКВЕНИРОВАНИЯ ILLUMINA И NANOPORE НА ПРИМЕРЕ НЕФТЯНЫХ ОБРАЗЦОВ. In: ВЕСТНИК СУРГУ. МЕДИЦИНА. 2025 ; Vol. 18, No. 2. pp. 81-87.

BibTeX

@article{5f385fd3d020440c8bfa43dfc03312f9,
title = "СРАВНЕНИЕ МЕТОДОВ ТАКСОНОМИЧЕСКОГО ОПРЕДЕЛЕНИЯ БАКТЕРИЙ ПО ПОСЛЕДОВАТЕЛЬНОСТИ ГЕНА 16S С ИСПОЛЬЗОВАНИЕМ ТЕХНОЛОГИЙ СЕКВЕНИРОВАНИЯ ILLUMINA И NANOPORE НА ПРИМЕРЕ НЕФТЯНЫХ ОБРАЗЦОВ",
abstract = "The use of modern sequencing methods enables us to determine the taxonomic biodiversity of various bacterial communities. Specifically, it allows to assess the diversity of bacterial strains that are not cultured. At present, there are two generations of sequencing technologies that enable the determination of microbial community diversity: 2nd generation (represented by Illumina and BGI) and 3rd generation (Nanopore and PacBio). However, as these technologies use different approaches to sequencing, there is a question of reproducibility of the results. This research revealed that using Illumina and Nanopore sequencing methods differs in terms of diversity indices and taxonomic diversity. This data emphasizes the requirement to develop advanced methods for sample preparation and DNA sequencing to achieve an unequivocal microbial community profile.",
author = "Бурлаченко, {Анастасия Сергеевна} and Некрасова, {Дарья Алексеевна} and Донников, {Михаил Юрьевич} and Ермаков, {Артем Михайлович} and Ждянова, {Елизавета Сергеевна} and Коваленко, {Людмила Васильевна} and Данилов, {Лаврентий Глебович} and Глотов, {Олег Сергеевич}",
year = "2025",
doi = "10.35266/2949-3447-2025-2-11",
language = "русский",
volume = "18",
pages = "81--87",
journal = "ВЕСТНИК СУРГУ. МЕДИЦИНА",
issn = "2304-9448",
publisher = "Сургутский государственный университет",
number = "2",

}

RIS

TY - JOUR

T1 - СРАВНЕНИЕ МЕТОДОВ ТАКСОНОМИЧЕСКОГО ОПРЕДЕЛЕНИЯ БАКТЕРИЙ ПО ПОСЛЕДОВАТЕЛЬНОСТИ ГЕНА 16S С ИСПОЛЬЗОВАНИЕМ ТЕХНОЛОГИЙ СЕКВЕНИРОВАНИЯ ILLUMINA И NANOPORE НА ПРИМЕРЕ НЕФТЯНЫХ ОБРАЗЦОВ

AU - Бурлаченко, Анастасия Сергеевна

AU - Некрасова, Дарья Алексеевна

AU - Донников, Михаил Юрьевич

AU - Ермаков, Артем Михайлович

AU - Ждянова, Елизавета Сергеевна

AU - Коваленко, Людмила Васильевна

AU - Данилов, Лаврентий Глебович

AU - Глотов, Олег Сергеевич

PY - 2025

Y1 - 2025

N2 - The use of modern sequencing methods enables us to determine the taxonomic biodiversity of various bacterial communities. Specifically, it allows to assess the diversity of bacterial strains that are not cultured. At present, there are two generations of sequencing technologies that enable the determination of microbial community diversity: 2nd generation (represented by Illumina and BGI) and 3rd generation (Nanopore and PacBio). However, as these technologies use different approaches to sequencing, there is a question of reproducibility of the results. This research revealed that using Illumina and Nanopore sequencing methods differs in terms of diversity indices and taxonomic diversity. This data emphasizes the requirement to develop advanced methods for sample preparation and DNA sequencing to achieve an unequivocal microbial community profile.

AB - The use of modern sequencing methods enables us to determine the taxonomic biodiversity of various bacterial communities. Specifically, it allows to assess the diversity of bacterial strains that are not cultured. At present, there are two generations of sequencing technologies that enable the determination of microbial community diversity: 2nd generation (represented by Illumina and BGI) and 3rd generation (Nanopore and PacBio). However, as these technologies use different approaches to sequencing, there is a question of reproducibility of the results. This research revealed that using Illumina and Nanopore sequencing methods differs in terms of diversity indices and taxonomic diversity. This data emphasizes the requirement to develop advanced methods for sample preparation and DNA sequencing to achieve an unequivocal microbial community profile.

UR - https://www.mendeley.com/catalogue/5c6f2c74-2300-3778-9fa7-ac39ed79e521/

U2 - 10.35266/2949-3447-2025-2-11

DO - 10.35266/2949-3447-2025-2-11

M3 - статья

VL - 18

SP - 81

EP - 87

JO - ВЕСТНИК СУРГУ. МЕДИЦИНА

JF - ВЕСТНИК СУРГУ. МЕДИЦИНА

SN - 2304-9448

IS - 2

ER -

ID: 137823903