The content of total, including structural and storage, lipids and fatty acids (FA) in the liver and gonads of females of the three-spined stickleback Gasterosteus aculeatus from different spawning grounds of the White Sea - the Seldjanaja Bay and the Kolyushkova Lagoon (belonged to Kandalaksha Bay), and also the Konyukhova Bay (belonged to Onega Bay). High content of total lipids in gonads (from 14.25 to 20.05% dry matter) was found, while significant differences in their content, as well as in the quantitative profile of the main lipid classes, may indicate differences in the characteristics of spawning. Different amount of structural phospholipids has been studied in the gonads, but the most variations were mostly related to the reserve triacylglycerols, and their components in form of two essential polyunsaturated FA – 22:6n-3, docosahexaenoic, and 20:5n-3, eicosapentaenoic, and monounsaturated – 16:1n-7, palmitoleic. In the gonads of fishes from the Konyukhova Bay, the highest level of reserve triacylglycerols and palmitoleic acid was detected, which can positively affect the body weight and viability of future embryos. The increased content of physiologically significant n-3 polyenoic acids (22:6n-3 and 20:5n-3) in the gonads of the stickleback females at this stage of the reproductive cycle may be associated with the increasing need for future embryos in these acids.