TY - JOUR

T1 - Применение дисперсионной жидкостно-жидкостной микроэкстракции с использованием в качестве экстрагента 3-метил-1-октил-имидазолий тетрафторбората для электрофоретического определения стероидных гормонов в образцах мочи

AU - Kolobova, E. A.

AU - Kartsova, L. A.

PY - 2018

Y1 - 2018

N2 - In the current research, a method of electrophoretic determination of steroid hormones in urine sample with the use of dispersive liquid-liquid microextraction in imidazolium ionic liquid (IL) C8MImBF4 during the sample preparation step was proposed. Ionic liquids С6MImNTf2, С6MImBF4 С8MImBF4 were examined as extractants while acetone, methanol and acetonitrile were tested as dispersants. It was found that the IL nature influences the extraction of steroid hormones, i.e. the extraction degrees were raised when IL with BF4 - anion was used instead of NTf2, and when the length of alkyl radical in imidazolium cation was increased. It was also established that pH of water phase did not affect the steroid hormones extraction. The highest extraction degree values of analytes (69-93%) were obtained under the following condition: addition of 150 mkl of IL C8MImBF4 dissolved in 0.5 ml of acetone to 5 ml aqueous sample. The developed sample preparation technique was successfully applied to the determination of corticosteroid in urine. It was found that the high concentration of salt in the sample matrix favors the mass transfer of analyte to organic phase, and the extraction degrees in IL were increased. The preconcentration factors in sample preparation were 23-30; the achieved limits of quantification were 8-12 ng/ml.

AB - In the current research, a method of electrophoretic determination of steroid hormones in urine sample with the use of dispersive liquid-liquid microextraction in imidazolium ionic liquid (IL) C8MImBF4 during the sample preparation step was proposed. Ionic liquids С6MImNTf2, С6MImBF4 С8MImBF4 were examined as extractants while acetone, methanol and acetonitrile were tested as dispersants. It was found that the IL nature influences the extraction of steroid hormones, i.e. the extraction degrees were raised when IL with BF4 - anion was used instead of NTf2, and when the length of alkyl radical in imidazolium cation was increased. It was also established that pH of water phase did not affect the steroid hormones extraction. The highest extraction degree values of analytes (69-93%) were obtained under the following condition: addition of 150 mkl of IL C8MImBF4 dissolved in 0.5 ml of acetone to 5 ml aqueous sample. The developed sample preparation technique was successfully applied to the determination of corticosteroid in urine. It was found that the high concentration of salt in the sample matrix favors the mass transfer of analyte to organic phase, and the extraction degrees in IL were increased. The preconcentration factors in sample preparation were 23-30; the achieved limits of quantification were 8-12 ng/ml.

KW - Capillary electrophoresis

KW - Dispersive liquid-liquid microextraction

KW - Imidazolium ionic liquids

KW - Liquid-liquid microextraction

KW - Steroid hormones

UR - http://www.scopus.com/inward/record.url?scp=85053928066&partnerID=8YFLogxK

U2 - 10.15826/analitika.2018.22.3.011

DO - 10.15826/analitika.2018.22.3.011

M3 - статья

AN - SCOPUS:85053928066

VL - 22

SP - 284

EP - 291

JO - АНАЛИТИКА И КОНТРОЛЬ

JF - АНАЛИТИКА И КОНТРОЛЬ

SN - 2073-1442

IS - 3

ER -