Objective: to analyze the parameters of 2D and 3D transthoracic echocardiography (TEE) with speckle-tracking technology in young athletes. Materials and methods: 79 athletes (52 male, 27 female, median age 20,5±3,7 years) undergone prospective analysis. Participants were divided into groups by level of training (group A-professionals and group B-amateurs) and by gender (group 1-men and group 2-women). Athletes underwent 12-lead ECG, 2D TTE and 3D TTE with a speckle-tracking. The evaluation of LV function was performed using the speckle-tracking method with the assessment of LV longitudinal strain in the Automated Function Imaging mode. Results: significant differences in LDV (р <0,0001), LAV (р <0,0001), LVEF (р <0,0001) between 2D (р<0,05) and 3D (р<0,0001) assessment, and differences in 2D (р <0,0001) and 3D (р=0,007) GLS values and in 3D GCS assessment (р=0,02) were registered. Significantly lower values of GLS were obtained in the group A in 2D (р=0,0002) and 3D (р=0,007) echo. Conclusions: 3D visualization with speckle-tracking allows careful and detailed assessment of left ventricular systolic function. The usage of left ventricle deformation quantitative analysis can detect early signs of systolic dysfunction with normal ejection fraction with a great value in young athletes.